Schiaffino S, Severin E, Hanzlíková V
Virchows Arch B Cell Pathol Incl Mol Pathol. 1979 Oct;31(2):169-79. doi: 10.1007/BF02889934.
Heart and skeletal muscle from rats of different ages were incubated in vitro in an oxygen-free medium supplied with substrates in order to investigate the effect of anoxia on muscle fine structure, particulary on the mitochondria. In skeletal muscle fibers anoxia has been found to induce changes similar to those previously described in ischemic muscles in vivo namely giant mitochondria, apparently derived by mitochondrial fusion, and intermembrane inclusions with a paracrystalline structure. The plate-like inclusions are mostly located in the intracristal spaces and are closely associated to cristal membranes even in markedly swollen mitochondria. Identical inclusions have been observed in cardiac muscle cells following anoxic injury, whereas they are never found in non-muscle cells such as endothelia, fibroblasts and nerve fibers. Cardiac and skeletal muscle fibers from newborn rats maintained in an oxygen-free medium show mitochondrial swelling but no intermembrane inclusions. The different response of mitochondria from developing vs adult striated muscle to anoxia may be due to changes during postnatal development in the quality or quantity of the protein component(s) involved in paracrystal formation.
为了研究缺氧对肌肉精细结构，特别是对线粒体的影响，将来自不同年龄大鼠的心脏和骨骼肌在体外于无氧培养基中培养，培养基中添加了底物。在骨骼肌纤维中，已发现缺氧会诱导产生与先前在体内缺血肌肉中所描述的类似变化，即巨大线粒体，显然是由线粒体融合形成的，以及具有类晶体结构的膜间内含物。板状内含物大多位于嵴内间隙，即使在线粒体明显肿胀时也与嵴膜紧密相关。在缺氧损伤后的心肌细胞中也观察到了相同的内含物，而在内皮细胞、成纤维细胞和神经纤维等非肌肉细胞中从未发现过。维持在无氧培养基中的新生大鼠的心肌和骨骼肌纤维显示出线粒体肿胀，但没有膜间内含物。发育中的横纹肌与成年横纹肌的线粒体对缺氧的不同反应可能是由于出生后发育过程中参与类晶体形成的蛋白质成分的质量或数量发生了变化。
