Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Neurochem Res. 2011 Dec;36(12):2427-33. doi: 10.1007/s11064-011-0570-7. Epub 2011 Aug 13.
This study aims to investigate the retinal metabolic processes in a rat axotomy model. Retinal metabolic changes in optic nerve transection (ONT) rat model were analyzed by (1)H magnetic resonance spectroscopy ((1)H-MRS). Retinal ganglion cells (RGCs) densities were assessed from retinal whole mounts. The retina was stained immunohistochemically with glial fibrillary acidic protein (GFAP). The results showed that the retina in ONT rats had significantly decreased concentrations of γ-aminobutyric acid (GABA), N-acetylaspartate (NAA), taurine (Tau), creatine (Cr) and increased concentrations of alanine (Ala) compared with control. Examination of glutamate (Glu), glutamine (Gln) and Glx (Glu + Gln) concentrations disclosed no significant differences. The mean density of RGCs reduced from 2,249 ± 87 cells/mm(2) in control group to 320 ± 56 cells/mm(2) in ONT group. GFAP immunoreactivity was markedly higher in ONT group than that in control group. The retinal metabolism after ONT was associated with neurotransmitter recycling/production perturbation, as well as other metabolic disequilibrium.
本研究旨在探讨大鼠视神经切断模型中的视网膜代谢过程。通过（1）H 磁共振波谱（1H-MRS）分析视神经横断（ONT）大鼠模型中的视网膜代谢变化。从视网膜全层铺片中评估视网膜神经节细胞（RGC）密度。用胶质纤维酸性蛋白（GFAP）对视网膜进行免疫组织化学染色。结果表明，与对照组相比，ONT 大鼠视网膜中γ-氨基丁酸（GABA）、N-乙酰天门冬氨酸（NAA）、牛磺酸（Tau）、肌酸（Cr）的浓度显著降低，而丙氨酸（Ala）的浓度显著升高。谷氨酸（Glu）、谷氨酰胺（Gln）和 Glx（Glu+Gln）浓度的检测没有显示出显著差异。RGC 密度从对照组的 2249±87 个/平方毫米降至 ONT 组的 320±56 个/平方毫米。ONT 组的 GFAP 免疫反应性明显高于对照组。ONT 后视网膜代谢与神经递质的再循环/产生失调以及其他代谢失衡有关。
