Institute of Applied Physics, University of Muenster, Corrensstraße 2/4 Muenster 48149, Germany.
J Biophotonics. 2012 Jan;5(1):9-13. doi: 10.1002/jbio.201100052. Epub 2011 Aug 15.
A reliable description and quantification of the complex physiology and reactions of living cells requires a multimodal analysis with various measurement techniques. We have investigated the integration of different techniques into a biophotonic workstation that can provide biological researchers with these capabilities. The combination of a micromanipulation tool with three different imaging principles is accomplished in a single inverted microscope which makes the results from all the techniques directly comparable. Chinese Hamster Ovary (CHO) cells were manipulated by optical tweezers while the feedback was directly analyzed by fluorescence lifetime imaging, digital holographic microscopy and dynamic phase-contrast microscopy.
要可靠地描述和量化活细胞的复杂生理学和反应，需要结合多种测量技术进行多模态分析。我们研究了将不同技术集成到一个生物光子工作站中，该工作站可为生物研究人员提供这些功能。在单个倒置显微镜中完成了微操作工具与三种不同成像原理的结合，这使得所有技术的结果都可以直接进行比较。通过光学镊子对中国仓鼠卵巢（CHO）细胞进行操作，而反馈则直接通过荧光寿命成像、数字全息显微镜和动态相衬显微镜进行分析。
