Center for Biomedical Optics and Photonics, University of Muenster, Robert-Koch-Str. 45, 48149 Muenster, Germany.
J Biophotonics. 2013 Mar;6(3):260-6. doi: 10.1002/jbio.201200057. Epub 2012 Jun 15.
The analysis of dynamic interactions of microorganisms with a host cell is of utmost importance for understanding infection processes. We present a biophotonic holographic workstation that allows optical manipulation of bacteria by holographic optical tweezers and simultaneously monitoring of dynamic processes with quantitative multi-focus phase imaging based on self-interference digital holographic microscopy. Our results show that several bacterial cells, even with non-spherical shape, can be aligned precisely on the surface of living host cells and localized reproducibly in three dimensions. In this way a new label-free multipurpose device for modelling and quantitative analysis of infection scenarios at the single cell level is provided.
分析微生物与宿主细胞的动态相互作用对于理解感染过程至关重要。我们提出了一种生物光子学全像工作站，该工作站允许通过全像光镊对细菌进行光学操纵，并同时利用基于自干涉数字全像显微术的定量多焦点相位成像进行动态过程的监测。我们的结果表明，即使是非球形的多个细菌细胞也可以精确地排列在活宿主细胞的表面上，并在三维空间中可重复地定位。通过这种方式，提供了一种新的无标记多功能设备，可用于在单细胞水平上对感染情况进行建模和定量分析。
