Institute for Applied Physics, Westfälische Wilhelms-Universität, Münster, Germany.
J Biophotonics. 2010 Jul;3(7):468-75. doi: 10.1002/jbio.201000033.
The class of rod-shaped bacteria is an important example of non-spherical objects where defined alignment is desired for the observation of intracellular processes or studies of the flagella. However, all available methods for orientational control of rod-shaped bacteria are either limited with respect to the accessible rotational axes or feasible angles or restricted to one single bacterium. In this paper we demonstrate a scheme to orientate rod-shaped bacteria with holographic optical tweezers (HOT) in any direction. While these bacteria have a strong preference to align along the direction of the incident laser beam, our scheme provides for the first time full rotational control of multiple bacteria with respect to any arbitrary axis. In combination with the translational control HOT inherently provide, this enables full control of all three translational and the two important rotational degrees of freedom of multiple rod-shaped bacteria and allows one to arrange them in any desired configuration.
杆状菌的分类是一种重要的非球形物体的例子，在观察细胞内过程或研究鞭毛时需要对其进行定向排列。然而，目前所有用于定向控制杆状菌的方法要么在可访问的旋转轴或可行角度上受到限制，要么只能用于单个细菌。在本文中，我们展示了一种使用全息光镊（HOT）将杆状菌定向到任意方向的方案。虽然这些细菌强烈倾向于沿着入射激光束的方向排列，但我们的方案首次提供了相对于任意轴的多个细菌的完全旋转控制。结合 HOT 固有的平移控制，这使得对多个杆状菌的所有三个平移自由度和两个重要的旋转自由度进行完全控制成为可能，并允许将它们排列在任何所需的构型中。
