Department of Mechanical and Aerospace Engineering, WVNano Initiative, West Virginia University, Morgantown, West Virginia 26506, United States.
Anal Chem. 2011 Sep 15;83(18):7061-5. doi: 10.1021/ac2019014. Epub 2011 Aug 26.
An ultrasensitive fluorescent sensor based on the quantum dot/DNA/gold nanoparticle ensemble has been developed for detection of mercury(II). DNA hybridization occurs when Hg(II) ions are present in the aqueous solution containing the DNA-conjugated quantum dots (QDs) and Au nanoparticles. As a result, the QDs and the Au nanoparticles are brought into the close proximity, which enables the nanometal surface energy transfer (NSET) from the QDs to the Au nanoparticles, quenching the fluorescence emission of the QDs. This nanosensor exhibits a limit of detection of 0.4 and 1.2 ppb toward Hg(II) in the buffer solution and in the river water, respectively. The sensor also shows high selectivity toward the Hg(II) ions.
一种基于量子点/DNA/金纳米粒子组装体的超灵敏荧光传感器已被开发用于检测汞（II）。当含有 DNA 偶联量子点 (QDs) 和 Au 纳米粒子的水溶液中存在 Hg(II)离子时，会发生 DNA 杂交。结果，QDs 和 Au 纳米粒子被带入近距离，从而使纳米金属表面能量转移 (NSET) 从 QDs 转移到 Au 纳米粒子，猝灭 QDs 的荧光发射。该纳米传感器在缓冲溶液和河水中对 Hg(II)的检测限分别为 0.4 和 1.2 ppb。该传感器对 Hg(II)离子也表现出高选择性。
