牛奶中小体细胞计数在评价临床健康和亚临床感染奶牛乳腺炎症反应中的应用。
Microscopic differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands.
机构信息
Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August-University Göttingen, Burckhardtweg 2, D-37077 Göttingen, Germany.
出版信息
J Dairy Res. 2011 Nov;78(4):448-55. doi: 10.1017/S0022029911000574. Epub 2011 Aug 16.
Somatic cell count (SCC) is generally regarded as an indicator of udder health. A cut-off value of 100×10(3) cells/ml is currently used in Germany to differentiate between normal and abnormal secretion of quarters. In addition to SCC, differential cell counts (DCC) can be applied for a more detailed analysis of the udder health status. The aim of this study was to differentiate somatic cells in foremilk samples of udder quarters classified as normal secreting by SCC <100×10(3) cells/ml. Twenty cows were selected and 72 normal secreting udder quarters were compared with a control group of six diseased quarters (SCC >100×10(3) cells/ml). In two severely diseased quarters of the control group (SCC of 967×10(3) cells/ml and 1824×10(3) cells/ml) Escherichia coli and Staphylococcus aureus were detected. DCC patterns of milk samples (n = 25) with very low SCC values of ≤6·25×10(3)cells/ml revealed high lymphocyte proportions of up to 92%. Milk cell populations in samples (n = 41) with SCC values of (>6·25 to ≤25)×10(3) cells/ml were also dominated by lymphocytes (mean value 47%), whereas DCC patterns of milk from udder quarters (n = 6) with SCC values (>25 to ≤100)×10(3)cells/ml changed. While in samples (n = 3) with SCC values of (27-33)×10(3) cells/ml macrophages were predominant (35-40%), three milk samples with (43-45)×10(3) cells/ml indicated already inflammatory reactions based on the predominance of polymorphonuclear leucocytes (PMN) (54-63%). In milk samples of diseased quarters PMN were categorically found as dominant cell population with proportions of ≥65%. Macrophages were the second predominant cell population in almost all samples tested in relationship to lymphocytes and PMN. To our knowledge, this is the first study evaluating cell populations in low SCC milk in detail. Udder quarters classified as normal secreting by SCC <100×10(3) cells/ml revealed already inflammatory processes based on DCC.
体细胞计数（SCC）通常被认为是乳房健康的指标。目前，德国使用 100×10（3）个细胞/ml 的截止值来区分正常和异常分泌的乳房 quarters。除了 SCC 之外，还可以应用差异细胞计数（DCC）来更详细地分析乳房健康状况。本研究的目的是区分 SCC<100×10（3）个细胞/ml 的正常分泌乳 quarters 中的体细胞。选择了 20 头奶牛，并将 72 个正常分泌的乳房 quarters 与对照组的 6 个患病 quarters（SCC>100×10（3）个细胞/ml）进行了比较。在对照组的两个严重患病 quarters 中（SCC 为 967×10（3）个细胞/ml 和 1824×10（3）个细胞/ml），检测到大肠杆菌和金黄色葡萄球菌。SCC 值非常低（≤6.25×10（3）个细胞/ml）的 25 份牛奶样本的 DCC 模式显示高达 92%的淋巴细胞比例。SCC 值为（>6.25 至≤25）×10（3）个细胞/ml 的 41 份样本的牛奶细胞群也主要由淋巴细胞（平均值 47%）组成，而 SCC 值为（>25 至≤100）×10（3）个细胞/ml 的 6 个乳房 quarters 的牛奶的 DCC 模式发生了变化。在 SCC 值为（27-33）×10（3）个细胞/ml 的 3 个样本中，巨噬细胞占主导地位（35-40%），而在 SCC 值为（43-45）×10（3）个细胞/ml 的 3 个样本中，基于多形核白细胞（PMN）的优势，已经存在炎症反应（PMN 为 54-63%）。在患病 quarters 的牛奶样本中，PMN 被确定为占主导地位的细胞群，比例≥65%。在与淋巴细胞和 PMN 相关的几乎所有测试样本中，巨噬细胞是第二主要的细胞群。据我们所知，这是首次详细评估 SCC 低的牛奶中的细胞群体。SCC<100×10（3）个细胞/ml 分类为正常分泌的乳房 quarters 已经根据 DCC 显示出炎症过程。
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