Iowa State University, Dept. of Entomology, Ames, IA 50011, USA.
J Invertebr Pathol. 2011 Oct;108(2):126-30. doi: 10.1016/j.jip.2011.07.004. Epub 2011 Aug 6.
Phagocytosis of pathogens by hemocytes is a rapid-acting immune response and represents a primary means of limiting microbial infection in some species of arthropods. To survey the relative capacity of hemocyte phagocytosis as a function of the arthropod immune response, we examined the extent of phagocytosis among a wide taxonomic range of arthropod species including a decapod crustacean (Litopenaeus vannamei), three ixodid tick species (Amblyomma americanum, Dermacentor variabilis, and Ixodes scapularis), a mosquito species (Aedes aegypti), and a larval moth (Manduca sexta). Injected fluorescent beads were used as a model to elicit phagocytosis and were measured by flow cytometry, a technique provided in detail that may be adapted for use with any species of arthropod. The data indicated that smaller arthropods generally had a higher proportion of phagocytic cells than larger arthropods.
血细胞吞噬病原体是一种快速的免疫反应，代表了某些节肢动物物种限制微生物感染的主要手段。为了调查血细胞吞噬作用作为节肢动物免疫反应的功能的相对能力，我们检查了广泛的节肢动物物种（包括十足目甲壳动物（凡纳滨对虾），三种硬蜱物种（美洲钝缘蜱、草原革蜱和肩突硬蜱），一种蚊子（埃及伊蚊）和一种幼虫蛾（烟草天蛾））的吞噬作用程度。注射荧光珠被用作引发吞噬作用的模型，并通过流式细胞术进行测量，详细介绍了一种可能适用于任何节肢动物物种的技术。数据表明，较小的节肢动物通常比较大的节肢动物具有更高比例的吞噬细胞。
