Department of Heavy Ion Radiation Biology and Medicine, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China.
Mutat Res. 2011 Nov 1;716(1-2):20-6. doi: 10.1016/j.mrfmmm.2011.07.016. Epub 2011 Aug 5.
The whole bodies of 6-week-old male Kun-Ming mice were exposed to different doses of (12)C(6+) ions or X-rays. Chromosomal aberrations of the bone marrow (gaps, terminal deletions and breaks, fragments, inter-chromosomal fusions and sister-chromatid union) were scored in metaphase 9h after exposure, corresponding to cells exposed in the G(2)-phase of the first mitosis cycle. Dose-response relationships for the frequency of chromosomal aberrations were plotted both by linear and linear-quadratic equations. The data showed that there was a dose-related increase in the frequency of chromosomal aberrations in all treated groups compared to controls. Linear-quadratic equations were a good fit for both radiation types. The compound theory of dual radiation action was applied to decipher the bigger curvature (D(2)) of the dose-response curves of X-rays compared to those of (12)C(6+) ions. Different distributions of the five types of aberrations and different degrees of homogeneity were found between (12)C(6+) ion and X-ray irradiation and the possible underlying mechanism for these phenomena were analyzed according to the differences in the spatial energy deposition of both types of radiation.
将 6 周龄雄性昆明种小鼠的全身体暴露于不同剂量的（12）C（6+）离子或 X 射线下。在暴露后 9 小时的有丝分裂中期，对骨髓（染色体断裂、染色体末端缺失、染色体片段、染色体间融合和姐妹染色单体交换）的染色体畸变进行评分，对应于第一有丝分裂周期 G2 期暴露的细胞。通过线性和线性二次方程绘制染色体畸变频率的剂量反应关系图。结果表明，与对照组相比，所有处理组的染色体畸变频率均呈剂量相关性增加。线性二次方程对两种辐射类型都有很好的拟合。双辐射作用复合理论被应用于解释 X 射线的剂量反应曲线的曲率（D2）大于（12）C6+离子的曲率。在（12）C6+离子和 X 射线照射之间发现了五种类型的畸变的不同分布和不同程度的均匀性，并且根据两种辐射类型的空间能量沉积的差异，分析了这些现象的可能潜在机制。
