Dept. of Anatomy, Histology, Forensic Medicine and Orthopedics, Section of Histology and Embryology, School of Pharmacy and Medicine, Sapienza University of Rome, Rome, Italy.
Mol Cell Endocrinol. 2012 Jan 2;348(1):135-46. doi: 10.1016/j.mce.2011.07.050. Epub 2011 Aug 5.
We have studied the effects of HGF on BTB dynamics in adult rats. We demonstrate that, at stages VII-VIII of the epithelium wave when germ cells traverse the BTB, HGF reduces the levels of occludin and influences its distribution pattern and assembling. Moreover, we report that, at stages VII-VIII, HGF significantly increases the amount of active TGF-β and the amount of uPA present in the tubules. For the first time we report that, in the same stages, HGF reduces the amount of actin present in the BTB region, in which occludin levels are highest, and modifies the morphology of the actin cytoskeleton network. At the level of maximal intensity of occludin fluorescence, we report that HGF also modifies the colocalization of occludin and actin. Lastly, we demonstrate that HGF is maximally expressed at stages VII-VIII, whereas its levels fall in the subsequent stages.
我们研究了 HGF 对成年大鼠 BTB 动态的影响。我们证明，在生精细胞穿越 BTB 的上皮波 VII-VIII 阶段，HGF 降低了 occludin 的水平，并影响了其分布模式和组装。此外，我们报告说，在 VII-VIII 阶段，HGF 显著增加了小管中活性 TGF-β 和 uPA 的量。我们首次报道，在同一阶段，HGF 减少了 occludin 水平最高的 BTB 区域中的肌动蛋白含量，并改变了肌动蛋白细胞骨架网络的形态。在 occludin 荧光强度最大的水平，我们报告说 HGF 也改变了 occludin 和肌动蛋白的共定位。最后，我们证明 HGF 在 VII-VIII 阶段表达最高，而在随后的阶段其水平下降。
