Key Laboratory of Coastal Zone Environment Processes, CAS, Shandong Provincial Key Laboratory of Coastal Zone Environment Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China.
Environ Toxicol Pharmacol. 2011 Sep;32(2):218-25. doi: 10.1016/j.etap.2011.05.006. Epub 2011 May 30.
Benzo(a)pyrene is an important polycyclic aromatic hydrocarbon (PAH) which causes carcinogenic, teratogenic and mutagenic effects in various species and the level of contamination of this toxic agent in the marine environment is of great concern. In this study, metabolic responses induced by two doses (0.02 and 0.2μM) of BaP were characterized in the gill tissues of Manila clam Ruditapes philippinarum after exposure for 24, 48 and 96h. The high dose (0.2μM) of BaP induced the disturbances in energy metabolism and osmotic regulation based on the metabolic biomarkers such as succinate, alanine, glucose, glycogen, branched chain amino acids, betaine, taurine, homarine, and dimethylamine in clam gills after 24h of exposure. In addition, hormesis induced by BaP was found in clams exposed to both doses of BaP. Overall, our results demonstrated the applicability of metabolomics for the elucidation of toxicological effects of marine environmental contaminants in a selected bioindicator species such as the Manila clam.
苯并(a)芘是一种重要的多环芳烃(PAH)，它会对各种物种造成致癌、致畸和致突变的影响，这种有毒物质在海洋环境中的污染水平令人高度关注。在这项研究中，研究了菲律宾蛤仔(Ruditapes philippinarum)鳃组织在暴露于苯并(a)芘两种剂量(0.02 和 0.2μM)24、48 和 96 小时后引起的代谢反应。高剂量(0.2μM)的苯并(a)芘在暴露 24 小时后，通过鳃中的琥珀酸、丙氨酸、葡萄糖、糖原、支链氨基酸、甜菜碱、牛磺酸、高肌氨酸、二甲胺等代谢生物标志物，引起能量代谢和渗透调节的紊乱。此外，还发现苯并(a)芘在两种剂量的苯并(a)芘暴露的蛤仔中诱导了毒物兴奋效应。总的来说，我们的研究结果表明，代谢组学可用于阐明海洋环境污染物对选定生物标志物物种(如菲律宾蛤仔)的毒理学影响。
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