Orthopaedic Oncology Institute of Chinese PLA, Tangdu Hospital, Xi-an, China.
Exp Biol Med (Maywood). 2011 Sep;236(9):1030-5. doi: 10.1258/ebm.2011.010315. Epub 2011 Aug 15.
Bladder cancer-associated protein (BLCAP) is a novel candidate tumor suppressor gene identified from human bladder carcinoma and highly associated with the invasion of bladder cancer. We previously reported that it also plays a key role in the tumorigenesis and metastasis of human osteosarcoma. In the present study, we constructed a recombinant encoding BLCAP cDNA. Overexpression of BLCAP resulted in growth inhibition and induced apoptosis of human TC-135 Ewing's sarcoma cells in vitro. We further investigated the caspase-3/7 activity and expressions of the fusion transcription factor Ewing's sarcoma protein-friend leukemia virus integration 1 (EWS-FLI1) and the apoptosis regulator B-cell lymphoma 2 (BCL-2). Cell apoptosis was accompanied by the down-regulated expression of EWS-FLI1 and BCL-2. Our present results suggest that BLCAP may play a role not only in regulating cell proliferation but also in coordinating apoptosis through the down-regulation of BCL-2 and EWS-FLI1 in human Ewing's sarcoma cells.
膀胱癌相关蛋白（BLCAP）是一种从人膀胱癌中鉴定出的新型候选肿瘤抑制基因，与膀胱癌的侵袭高度相关。我们之前的报告表明，它在人类骨肉瘤的发生和转移中也起着关键作用。在本研究中，我们构建了一个编码 BLCAP cDNA 的重组体。BLCAP 的过表达导致体外人 TC-135 尤文肉瘤细胞的生长抑制和诱导凋亡。我们进一步研究了半胱天冬酶-3/7 活性以及融合转录因子尤文肉瘤蛋白-友白血病病毒整合 1（EWS-FLI1）和凋亡调节因子 B 细胞淋巴瘤 2（BCL-2）的表达。细胞凋亡伴随着 EWS-FLI1 和 BCL-2 的下调表达。我们目前的结果表明，BLCAP 不仅可能通过下调 BCL-2 和 EWS-FLI1 来调节细胞增殖，而且可能通过协调凋亡来发挥作用，从而在人尤文肉瘤细胞中发挥作用。
