miR-290-295 缺失的小鼠表现为部分显性胚胎致死和生殖细胞缺陷。
Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects.
机构信息
Whitehead Institute for Biomedical Research, Cambridge, MA 02139, USA.
出版信息
Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14163-8. doi: 10.1073/pnas.1111241108. Epub 2011 Aug 15.
Abstract
Mir-290 through mir-295 (mir-290-295) is a mammalian-specific microRNA (miRNA) cluster that, in mice, is expressed specifically in early embryos and embryonic germ cells. Here, we show that mir-290-295 plays important roles in embryonic development as indicated by the partially penetrant lethality of mutant embryos. In addition, we show that in surviving mir-290-295-deficient embryos, female but not male fertility is compromised. This impairment in fertility arises from a defect in migrating primordial germ cells and occurs equally in male and female mutant animals. Male mir-290-295(-/-) mice, due to the extended proliferative lifespan of their germ cells, are able to recover from this initial germ cell loss and are fertile. Female mir-290-295(-/-) mice are unable to recover and are sterile, due to premature ovarian failure.
摘要
miR-290 到 miR-295（miR-290-295）是哺乳动物特异性 microRNA（miRNA）簇，在小鼠中，其特异性在早期胚胎和胚胎生殖细胞中表达。在这里，我们表明，miR-290-295 在胚胎发育中起着重要作用，突变胚胎的部分穿透致死性表明了这一点。此外，我们表明，在存活的 miR-290-295 缺陷胚胎中，雌性但不是雄性的生育能力受到损害。这种生育能力的损害源于迁移原始生殖细胞的缺陷，并且在雄性和雌性突变动物中同样发生。由于其生殖细胞的延长增殖寿命，雄性 miR-290-295（-/-）小鼠能够从这种初始生殖细胞损失中恢复，并且具有生育能力。由于卵巢早衰，雌性 miR-290-295（-/-）小鼠无法恢复，并且不育。
相似文献
1
Mir-290-295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects.miR-290-295 缺失的小鼠表现为部分显性胚胎致死和生殖细胞缺陷。
Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14163-8. doi: 10.1073/pnas.1111241108. Epub 2011 Aug 15.
2
Potential role of miR-29b in modulation of Dnmt3a and Dnmt3b expression in primordial germ cells of female mouse embryos.miR-29b在调节雌性小鼠胚胎原始生殖细胞中Dnmt3a和Dnmt3b表达方面的潜在作用。
RNA. 2009 Aug;15(8):1507-14. doi: 10.1261/rna.1418309. Epub 2009 Jun 9.
3
Loss of Apela Peptide in Mice Causes Low Penetrance Embryonic Lethality and Defects in Early Mesodermal Derivatives.小鼠中Apela肽缺失导致低发生率的胚胎致死性及早期中胚层衍生物缺陷。
Cell Rep. 2017 Aug 29;20(9):2116-2130. doi: 10.1016/j.celrep.2017.08.014.
4
Ablation of the MiR-17-92 MicroRNA Cluster in Germ Cells Causes Subfertility in Female Mice.生殖细胞中MiR-17-92微小RNA簇的缺失导致雌性小鼠生育力低下。
Cell Physiol Biochem. 2018;45(2):491-504. doi: 10.1159/000487028. Epub 2018 Jan 25.
5
Heart defects and embryonic lethality in Asb2 knock out mice correlate with placental defects.Asb2基因敲除小鼠的心脏缺陷和胚胎致死率与胎盘缺陷相关。
Cells Dev. 2021 Mar;165:203663. doi: 10.1016/j.cdev.2021.203663. Epub 2021 Jan 27.
6
Partially penetrant postnatal lethality of an epithelial specific MicroRNA in a mouse knockout.上皮细胞特异性 MicroRNA 敲除小鼠的部分穿透性产后致死性。
PLoS One. 2013 Oct 7;8(10):e76634. doi: 10.1371/journal.pone.0076634. eCollection 2013.
7
Regulation of glucose phosphate isomerase by the 3'UTR-specific miRNAs miR-302b and miR-17-5p in chicken primordial germ cells.3'UTR 特异性 miRNAs miR-302b 和 miR-17-5p 对鸡原始生殖细胞中葡萄糖磷酸异构酶的调控作用。
Biol Reprod. 2013 Aug 15;89(2):33. doi: 10.1095/biolreprod.112.105692. Print 2013 Aug.
8
PUMA regulates germ cell loss and primordial follicle endowment in mice.PUMA调节小鼠生殖细胞损失和原始卵泡数量。
Reproduction. 2014 Aug;148(2):211-9. doi: 10.1530/REP-13-0666. Epub 2014 May 23.
9
RBX1/ROC1 disruption results in early embryonic lethality due to proliferation failure, partially rescued by simultaneous loss of p27.RBX1/ROC1功能破坏会因增殖失败导致早期胚胎致死，而p27的同时缺失可部分挽救这种情况。
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6203-8. doi: 10.1073/pnas.0812425106. Epub 2009 Mar 26.
10
MiR-302/367 regulate neural progenitor proliferation, differentiation timing, and survival in neurulation.微小RNA-302/367在神经胚形成过程中调节神经祖细胞的增殖、分化时间及存活。
Dev Biol. 2015 Dec 1;408(1):140-50. doi: 10.1016/j.ydbio.2015.09.020. Epub 2015 Oct 9.
引用本文的文献
1
Whole-transcriptome sequencing reveals the effects of acupuncture on early embryos post-IVF-ET in poor ovarian response.全转录组测序揭示针刺对卵巢反应不良患者体外受精-胚胎移植后早期胚胎的影响。
J Ovarian Res. 2025 Apr 30;18(1):91. doi: 10.1186/s13048-025-01682-7.
2
The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.miR-290和miR-302基因簇对于将成纤维细胞重编程为诱导多能干细胞至关重要。
Stem Cells. 2025 Feb 12;43(2). doi: 10.1093/stmcls/sxae080.
3
Analysis of a mouse germ cell tumor model establishes pluripotency-associated miRNAs as conserved serum biomarkers for germ cell cancer detection.对小鼠生殖细胞肿瘤模型的分析确定了多能性相关的微小RNA作为用于生殖细胞癌检测的保守血清生物标志物。
Sci Rep. 2025 Feb 6;15(1):4452. doi: 10.1038/s41598-025-88554-8.
4
The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.母源-合子转变：细胞质与细胞核的重编程
Nat Rev Genet. 2025 Apr;26(4):245-267. doi: 10.1038/s41576-024-00792-0. Epub 2024 Nov 25.
5
The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.miR-290和miR-302基因簇对于将成纤维细胞重编程为诱导多能干细胞至关重要。
bioRxiv. 2024 Sep 3:2024.09.02.610895. doi: 10.1101/2024.09.02.610895.
6
The Role of microRNA in Spermatogenesis: Is There a Place for Fertility Preservation Innovation?微小 RNA 在精子发生中的作用：是否有创新的生育力保存空间？
Int J Mol Sci. 2023 Dec 29;25(1):460. doi: 10.3390/ijms25010460.
7
Evidence for Functional Roles of MicroRNAs in Lineage Specification During Mouse and Human Preimplantation Development.在小鼠和人类胚胎植入前发育过程中 miRNA 在线粒体分化中的功能作用的证据。
Yale J Biol Med. 2023 Dec 29;96(4):481-494. doi: 10.59249/FOSI4358. eCollection 2023 Dec.
8
functions of miRNAs in mammalian spermatogenesis.微小RNA在哺乳动物精子发生中的功能。
Front Cell Dev Biol. 2023 May 5;11:1154938. doi: 10.3389/fcell.2023.1154938. eCollection 2023.
9
MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1.miR-145 在发育迟缓的胚胎中上调，并通过靶向 Abca1 调节小鼠胚胎着床前的胆固醇水平。
Reprod Biol Endocrinol. 2022 Dec 12;20(1):168. doi: 10.1186/s12958-022-01044-8.
10
Genetic Variations miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G and the Risk of Recurrent Pregnancy Loss in Korean Women.韩国女性中miR-10aA>T、miR-30cA>G、miR-181aT>C和miR-499bA>G基因变异与复发性流产风险
Biomedicines. 2022 Sep 25;10(10):2395. doi: 10.3390/biomedicines10102395.
本文引用的文献
1
A latent pro-survival function for the mir-290-295 cluster in mouse embryonic stem cells.miR-290-295 簇在小鼠胚胎干细胞中的潜在生存促进功能。
PLoS Genet. 2011 May;7(5):e1002054. doi: 10.1371/journal.pgen.1002054. Epub 2011 May 5.
2
miRNA regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration.miRNA 对 Sdf1 趋化因子信号的调节为生殖细胞迁移提供了遗传稳定性。
Nat Genet. 2011 Mar;43(3):204-11. doi: 10.1038/ng.758. Epub 2011 Jan 23.
3
MicroRNAs and gene regulatory networks: managing the impact of noise in biological systems.microRNAs 与基因调控网络：管理生物系统中的噪声影响。
Genes Dev. 2010 Jul 1;24(13):1339-44. doi: 10.1101/gad.1937010.
4
Variability in gene expression underlies incomplete penetrance.基因表达的可变性是不完全外显的基础。
Nature. 2010 Feb 18;463(7283):913-8. doi: 10.1038/nature08781.
5
The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection.miR-430/427/302家族通过物种特异性的靶标选择来控制中内胚层命运特化。
Dev Cell. 2009 Apr;16(4):517-27. doi: 10.1016/j.devcel.2009.02.007.
6
A microRNA imparts robustness against environmental fluctuation during development.一种微小RNA在发育过程中赋予对环境波动的稳健性。
Cell. 2009 Apr 17;137(2):273-82. doi: 10.1016/j.cell.2009.01.058.
7
Embryonic stem cell-specific microRNAs promote induced pluripotency.胚胎干细胞特异性微小RNA促进诱导多能性。
Nat Biotechnol. 2009 May;27(5):459-61. doi: 10.1038/nbt.1535. Epub 2009 Apr 12.
8
Attribution of vascular phenotypes of the murine Egfl7 locus to the microRNA miR-126.小鼠Egfl7基因座血管表型归因于微小RNA miR-126
Development. 2008 Dec;135(24):3989-93. doi: 10.1242/dev.029736. Epub 2008 Nov 5.
9
Embryonic stem cell-specific microRNAs regulate the G1-S transition and promote rapid proliferation.胚胎干细胞特异性微小RNA调控G1-S期转换并促进快速增殖。
Nat Genet. 2008 Dec;40(12):1478-83. doi: 10.1038/ng.250. Epub 2008 Nov 2.
10
A C. elegans genome-scale microRNA network contains composite feedback motifs with high flux capacity.秀丽隐杆线虫全基因组规模的微小RNA网络包含具有高通量能力的复合反馈基序。
Genes Dev. 2008 Sep 15;22(18):2535-49. doi: 10.1101/gad.1678608.
Zotero 插件