Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Iran.
Folia Neuropathol. 2011;49(2):142-51.
The aim of this study was to investigate the histological changes in the dorsal root ganglion (DRG) and the sciatic nerve in rats after sciatic nerve crush (SNC) and treatment with rosiglitazone. The rats were divided into four groups, each including seven animals, and underwent the following intervention. Group I: control animals which received carboxy methyl cellulose (0.5 w/v, p.o.). Group II: sham operated animals whose skin of the posterior thigh was opened, closed and the animals received the vehicle (carboxy methyl cellulose). Group III: SNC animals; the animals received the vehicle. Group IV: SNC with rosiglitazone (5 mg/kg body weight/day) dissolved in the vehicle. On the 28th day the fifth lumbar DRG and sciatic nerve were removed. Volume of the dorsal root ganglion, total volume and number of cells (A and B cells) of DRG, total surface area of the cells, and total number, diameter and cross-sectional area of the myelinated nerve fibres were estimated using stereological techniques. No change was observed in volume of the DRG, but all of the other parameters were decreased after nerve crush. In SNC+ rosiglitazone treated rats, the parameters decreased but to a lesser extent in comparison with the non-treated SNC group. It can be concluded that rosiglitazone has a protective effect on the DRG cells and sciatic nerve after crush in rats.
本研究旨在探讨坐骨神经挤压（SNC）后，罗格列酮治疗对大鼠背根神经节（DRG）和坐骨神经组织学变化的影响。将大鼠分为四组，每组 7 只，分别接受以下干预。组 I：给予羧甲基纤维素（0.5 w/v，口服）的对照组动物。组 II：仅切开、缝合后腿后侧皮肤的假手术组动物，给予载体（羧甲基纤维素）。组 III：SNC 动物；给予载体。组 IV：SNC 加罗格列酮（5mg/kg 体重/天），溶于载体。第 28 天取出第 5 腰椎 DRG 和坐骨神经。使用体视学技术评估 DRG 体积、DRG 总体积和细胞总数（A 和 B 细胞）、细胞总表面积、有髓神经纤维总数、直径和横截面积。DRG 体积无变化，但神经挤压后所有其他参数均降低。在 SNC+罗格列酮治疗组中，与未经治疗的 SNC 组相比，这些参数降低，但程度较轻。可以得出结论，罗格列酮对大鼠挤压后 DRG 细胞和坐骨神经具有保护作用。
