Histomorphometry & Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
Pathol Res Pract. 2011 Sep 15;207(9):577-82. doi: 10.1016/j.prp.2011.06.011. Epub 2011 Aug 5.
The goal of this study was to quantify the histological changes in the dorsal root ganglion (DRG) and the sciatic nerve in rats subjected to sciatic nerve crush (SNC) following curcumin treatment. The rats were divided into four groups, each including five animals, and underwent the following intervention: group I: control animals which received olive oil; group II: sham-operated animals whose skin of the posterior thigh was opened, sutured, and received the vehicle; group III: SNC animals which received the vehicle; and group IV: SNC plus curcumin (100 mg/kg/day) solved in the vehicle. On the 28th day, the fifth lumbar DRG and sciatic nerve were removed. Volume of the ganglion, mean cell volume, total volume of DRG cells (A- and B-cells), and total surface of DRG cells, total number, diameter, and area of the myelinated nerve fibers were estimated using stereological methods. Except for the volume of the ganglion, all other parameters were decreased after nerve crush. In curcumin-treated rats, these parameters decreased, but to a lesser extent, and the values were significantly higher than in the non-treated SNC group (p<0.04). It can be concluded that in rats after crush, curcumin has a protective effect on the DRG and sciatic nerve.
本研究的目的是定量分析姜黄素处理后坐骨神经挤压（SNC）大鼠背根神经节（DRG）和坐骨神经的组织学变化。将大鼠分为四组，每组 5 只，分别进行以下干预：I 组：对照组，给予橄榄油；II 组：假手术组，仅切开、缝合后大腿后侧皮肤，给予载体；III 组：SNC 组，给予载体；IV 组：SNC 加姜黄素（100mg/kg/天）溶于载体。第 28 天，取出第 5 腰椎 DRG 和坐骨神经。使用体视学法估计神经节体积、平均细胞体积、DRG 细胞总容积（A-和 B-细胞）、DRG 细胞总表面积、有髓神经纤维总数、直径和面积。除神经节体积外，所有其他参数在神经挤压后均降低。在姜黄素治疗的大鼠中，这些参数降低，但程度较轻，且与未治疗的 SNC 组相比，数值显著更高（p<0.04）。结论是，在大鼠挤压后，姜黄素对 DRG 和坐骨神经具有保护作用。
