Zhao Jing, Ruan Hong, Gao Qiu-ping, Li Meng-ya, Tao Ye-qi, Zheng Ying
School of Medicine and Life Sciences, Zhejiang University City College, Hangzhou 310015, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2011 Jul;40(4):365-73. doi: 10.3785/j.issn.1008-9292.2011.04.004.
To isolate and purify components from polysaccharides of purple sweet potato (PPSP) and to test their anti-tumor activity.
DEAE-Cellulose and CM-Cellulose exchange chromatography were applied to separate components of PPSP. The anti-tumor activities of each component were measured by MTT assay on Hela and HepG(2) cells and their monosaccharide composition were analyzed by TLC chromatography, followed by infrared spectroscopy studies.
Through weak anion exchange chromatography and gradient elution by sodium chloride solution, four components were separated and named as PPSP, PPSPII, PPSPIII and PPSPIV, respectively. MTT tests showed that PPSP II and PPSPIII inhibited Hela and HepG2 tumor cells in a certain extent. The structural analysis revealed that PPSPI was mainly composed of glucose and galactose, PPSP II was composed of glucose and had a typical absorption peak of β-D-glucose chitosan pyranose, PPSP III was a glycoprotein showing a protein absorption peak.
Four components were separated from PPSP successfully, among which PPSP II and PPSP III shows anti-tumor activities on Hela and HepG(2) cells in vitro.
从紫甘薯多糖（PPSP）中分离纯化各组分，并检测其抗肿瘤活性。
采用DEAE-纤维素和CM-纤维素交换色谱法分离PPSP各组分。通过MTT法检测各组分对Hela细胞和HepG(2)细胞的抗肿瘤活性，并用薄层色谱法分析其单糖组成，随后进行红外光谱研究。
通过弱阴离子交换色谱和氯化钠溶液梯度洗脱，分离出四个组分，分别命名为PPSP、PPSPII、PPSPIII和PPSPIV。MTT试验表明，PPSP II和PPSPIII在一定程度上抑制了Hela细胞和HepG2肿瘤细胞。结构分析表明，PPSPI主要由葡萄糖和半乳糖组成，PPSP II由葡萄糖组成，具有典型的β-D-葡萄糖壳聚糖吡喃糖吸收峰，PPSP III是一种显示蛋白质吸收峰的糖蛋白。
成功从PPSP中分离出四个组分，其中PPSP II和PPSP III在体外对Hela细胞和HepG(2)细胞具有抗肿瘤活性。
