Active Carbohydrate Research Institute, Chongqing Key Laboratory of Inorganic Functional Materials, Chongqing Normal University, Chongqing, China.
Chem Biol Drug Des. 2022 Jan;99(1):111-117. doi: 10.1111/cbdd.13935. Epub 2021 Sep 17.
Extraction and deproteinization process of polysaccharide from purple sweet potato (PPSP) were optimized via the response surface methodology (RSM). The results indicated that the optimal conditions of extraction in hot water of PPSP were as follows: The extraction temperature was 120℃, the extraction time was 2.5 hr, and the solid-liquid ratio was 1∶10 (g/ml). The optimal conditions of Sevage deproteinization were as under the oscillation time was 20 min, the deproteinization times was twice, and polysaccharide solution-Sevage reagent ratio was 1:1 (ml/ml). The extraction yield of PPSP was 3.32%, and the protein removal rate was 93.14% in such a condition.
通过响应面法（RSM）优化了紫薯（PPSP）多糖的提取和脱蛋白工艺。结果表明，PPSP 热水提取的最佳条件为：提取温度 120℃，提取时间 2.5 小时，固液比 1∶10（g/ml）。Sevage 脱蛋白的最佳条件为：振荡时间 20 分钟，脱蛋白次数 2 次，多糖溶液-Sevage 试剂比为 1:1（ml/ml）。在此条件下，PPSP 的提取率为 3.32%，蛋白质去除率为 93.14%。
