Institute for Research in Molecular Medicine (INFORMM), University Sains Malaysia, Penang, Malaysia.
Eur Rev Med Pharmacol Sci. 2011 Aug;15(8):875-82.
Candida (C.) albicans infection in its biofilm mode of growth has taken centre point with the increasing recognition of its role in human infections due to the development of resistance to the commonly used antibiotic or phenotypic adaptation within the biofilm. Hence, in this study the inhibitory effect of methanol extract of Cassia (C.) spectabilis leaves was evaluated against biofilm forming C. albicans.
Anti-yeast activities were carried out using disc diffusion assay and broth dilution method against biofilm forming C. albicans. C. spectabilis leaves extract was assessed using XTT (2,3-bis [2-Methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide) reduction assay for biofilm quantification with positive control fluconazole. Scanning electron microscopic (SEM) and confocal scanning laser microscopy (CLSM) analysis further revealed reduction in C. albicans biofilm by C. spectabilis leaves extract.
The methanol extract of C. spectabilis showed a favorable antiyeast activity against C. albicans with MIC (Minimum Inhibitory Concentration) value of 6.25 mg/ml. Fluconazole and leaves extract showed 95.4% and 96.9% biofilm reduction respectively. The main changes observed under scanning electron microscopy after C. spectabilis leaves extract treatment were cellular damage and disruption in biofilms of C. albicans. The ultrastructural changes visualized by SEM were further confirmed using CLSM study.
The results from this research conclusively exhibit the in vitro anti-biofilm potential of C. spectabilis leaves extract against Candida biofilm.
由于对常用抗生素的耐药性发展或生物膜内的表型适应，白色念珠菌（C.）生物膜生长方式的感染已成为关注焦点，其在人类感染中的作用日益得到认识。因此，本研究评估了决明子（C.）叶甲醇提取物对形成生物膜的白色念珠菌的抑制作用。
采用圆盘扩散法和肉汤稀释法对形成生物膜的白色念珠菌进行抗酵母活性检测。采用 XTT（2,3-双[2-甲氧基-4-硝基-5-磺苯基]-2H-四唑-5-羧基苯胺）还原法评估决明子叶提取物的生物膜定量，阳性对照氟康唑。扫描电子显微镜（SEM）和共聚焦扫描激光显微镜（CLSM）分析进一步显示决明子叶提取物减少了白色念珠菌生物膜。
决明子甲醇提取物对白色念珠菌表现出良好的抗酵母活性，最低抑菌浓度（MIC）值为 6.25mg/ml。氟康唑和叶片提取物分别显示出 95.4%和 96.9%的生物膜减少。决明子叶提取物处理后，在扫描电子显微镜下观察到的主要变化是白色念珠菌生物膜中的细胞损伤和破坏。通过 SEM 观察到的超微结构变化在 CLSM 研究中得到了进一步证实。
本研究结果明确显示了决明子叶提取物对念珠菌生物膜的体外抗生物膜潜力。
