松果菊不同提取物的抗菌和抗氧化活性（玄参科）。

Antimicrobial and antioxidant activities of the various extracts of Verbascum pinetorum Boiss. O. Kuntze (Scrophulariaceae).

机构信息

Biology Department, Faculty of Sciences and Letters, Mustafa Kemal University, Hatay, Turkey.

出版信息

Eur Rev Med Pharmacol Sci. 2011 Aug;15(8):900-5.

Abstract

BACKGROUND AND OBJECTIVES

The present study was performed to evaluate the in vitro antimicrobial and antioxidant properties of various extracts of Verbascum (V.) pinetorum, a member of Scrophulariaceae family. While the antimicrobial activity of various extracts of V. pinetorum was determined with agar-well diffusion method, the antioxidant activity was examined with two complementary test systems, namely 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and beta-carotene/linoleic acid test systems.

RESULTS

The hexane extract exhibits antimicrobial activity against few microorganisms. However, dichloromethane, direct methanol and methanol/chloroform extracts are effective on a broad range of microorganisms. Among the tested bacteria Haemophilus influenzae was found to be the most sensitive bacterium. The 50% (IC50) inhibition activity of the methanolic extract of V. pinetorum on the free radical DPPH was determined as 13.04 mg/ml. In the case of the linoleic acid system, oxidation of linoleic acid was inhibited by methanolic extract of V. pinetorum, which showed 89.39% inhibition that is quite close to the value of the synthetic antioxidant reagent butylhydroxytoluene (BHT), 92.46%. Iridoid glycosides, flavonoids and saponins were determined as the major natural compounds in the methanolic extracts. The total phenolic components of V. pinetorum were found as 42.45 mg/g gallic acid equivalent.

CONCLUSION

The results provide evidence that the extracts of V. pinetorum contained iridoid glycosides, flavonoids, saponins and phenolic compounds which may be responsible for the substantial antimicrobial and antioxidant activities.

摘要

背景与目的

本研究旨在评估玄参科（Scrophulariaceae）列当属（Verbascum）植物 pinetorum 的不同提取物的体外抗菌和抗氧化特性。在使用琼脂孔扩散法测定各种 V. pinetorum 提取物的抗菌活性的同时，还使用两种互补的测试系统来检查其抗氧化活性，即 1,1-二苯基-2-苦基肼（DPPH）自由基清除和β-胡萝卜素/亚油酸测试系统。

结果

正己烷提取物对少数微生物具有抗菌活性。然而，二氯甲烷、直接甲醇和甲醇/氯仿提取物对广泛的微生物有效。在所测试的细菌中，流感嗜血杆菌被发现是最敏感的细菌。V. pinetorum 甲醇提取物对自由基 DPPH 的 50%（IC50）抑制活性被确定为 13.04mg/ml。在亚油酸体系中，V. pinetorum 甲醇提取物抑制亚油酸的氧化，其抑制率达到 89.39%，与合成抗氧化剂叔丁基对苯二酚（BHT）92.46%的抑制率相当。在甲醇提取物中确定了环烯醚萜糖苷、类黄酮和皂苷为主要天然化合物。V. pinetorum 的总酚类成分被确定为 42.45mg/g 没食子酸当量。