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[按照国家标准制备及鉴定抗牛种布鲁氏菌血清]

[Preparation and characterization of follwing the national standard anti-Brucella abortus serum, bovine].

作者信息

Li Cui, Guan Fushi, Dai Zhihong, Jiang Hui, Wen Fang, Lu Lianshou, Wang Zaishi

机构信息

China Institute of Veterinary Drug Control, Beijing 100081, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2011 May;27(5):812-6.

Abstract

To prepare anti-Brucella abortus serum used for calibrate the agglutination test follwing the national standard, 4 anti-Brucella abortus sera were obtained from 4 cows infected with Brucella abortus naturally. By potency testing, the third serum was selected. Sterility, vaccum degree, residual moisture, uniformity and stability of this standard material were tested and proved to meet the national standard. Referring to the international standard, RBT (Rose-Bengal plate agglutination test), SAT (standard tube agglutination) and CFT (complement fixation test) titers of this standard material were measured to be 1:160 "+" 1:2 400 "++" and 1:800 "++", which are identical with the collaborative assay results. International unit of the standard material is 4 000 IU/mL.

摘要

为制备用于按照国家标准校准凝集试验的抗布鲁氏菌流产血清,从4头自然感染布鲁氏菌流产的奶牛中获取了4份抗布鲁氏菌流产血清。通过效价检测,选择了第三份血清。对该标准物质的无菌性、真空度、残留水分、均匀性和稳定性进行了检测,结果证明符合国家标准。参照国际标准,测定该标准物质的虎红平板凝集试验(RBT)、标准试管凝集试验(SAT)和补体结合试验(CFT)效价分别为1:160“+”、1:2400“++”和1:800“++”,与协作试验结果一致。该标准物质的国际单位为4000 IU/mL。

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