Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, Rio de Janeiro, Brazil.
J Endod. 2011 Sep;37(9):1206-12. doi: 10.1016/j.joen.2011.05.026. Epub 2011 Jul 16.
This molecular study analyzed the microbiota of primary root canal infections from adult Norwegian patients.
Samples were taken from the necrotic root canals of teeth with symptomatic (n = 13) or asymptomatic (n = 21) apical periodontitis and chronic apical abscesses (n = 9). DNA was extracted from samples, and bacterial identifications were performed by a closed-ended reverse-capture checkerboard approach targeting 50 candidate endodontic pathogens.
Bacterial DNA was detected in all cases. In teeth with asymptomatic apical periodontitis, the most frequent taxa were Dialister invisus (71%), Fusobacterium nucleatum (62%), and Porphyromonas endodontalis (62%). In chronic apical abscesses, the most prevalent taxa were P. endodontalis (100%), D. invisus (89%), Parvimonas micra (78%), and Solobacterium moorei (78%). In teeth with symptomatic apical periodontitis, the most prevalent taxa were D. invisus, P. endodontalis, S. moorei, Propionibacterium acnes, and Streptococcus species (all in 69%). None of the targeted taxa were significantly associated with either sinus tract or pain (P > .05), except for Selenomonas sputigena, which was more frequently found in painful cases (P = .04). No taxa were found in significantly higher levels in any conditions (P > .05). Cluster analyses revealed bacterial groupings that differed between cases with and without pain.
Although basically the same species were highly prevalent in the different conditions examined and none of the most prevalent taxa were positively associated with symptoms, results revealed that species formed different partnerships and associations in samples from teeth with or without pain. Therefore, it is possible that more virulent multispecies communities can form as a result of overall bacterial combinations and give rise to acute inflammation.
本分子研究分析了来自挪威成年患者原发性根管感染的微生物群。
从有症状（n = 13）或无症状（n = 21）根尖周炎和慢性根尖脓肿的牙齿的坏死根管中采集样本。从样本中提取 DNA，并通过针对 50 种候选牙髓病原体的封闭式反向捕获棋盘法进行细菌鉴定。
所有病例均检测到细菌 DNA。在无症状根尖周炎的牙齿中，最常见的类群是 Dialister invisus（71%）、Fusobacterium nucleatum（62%）和 Porphyromonas endodontalis（62%）。在慢性根尖脓肿中，最常见的类群是 P. endodontalis（100%）、D. invisus（89%）、Parvimonas micra（78%）和 Solobacterium moorei（78%）。在有症状的根尖周炎的牙齿中，最常见的类群是 D. invisus、P. endodontalis、S. moorei、Propionibacterium acnes 和 Streptococcus 属（均为 69%）。除了 Selenomonas sputigena 外，没有一种目标类群与窦道或疼痛有显著相关性（P >.05），Selenomonas sputigena 在疼痛病例中更为常见（P =.04）。在任何情况下，都没有发现任何类群的水平显著升高（P >.05）。聚类分析显示，在有疼痛和无疼痛的病例之间存在细菌分组差异。
尽管在不同条件下高度流行的基本上是相同的物种，而且没有一种最常见的类群与症状呈正相关，但结果表明，在有疼痛和无疼痛的牙齿样本中，物种形成了不同的伙伴关系和关联。因此，更具毒力的多物种群落可能由于整体细菌组合而形成，并引发急性炎症。
