Siqueira José F Júnior, Rôças Isabela N
Department of Endodontics, Faculty of Dentistry, Estácio de Sá University, Rio de Janeiro, Brazil.
J Endod. 2003 Jul;29(7):438-41. doi: 10.1097/00004770-200307000-00003.
Dialister pneumosintes is an anaerobic Gram-negative rod that has been recently implicated as a candidate endodontic pathogen. In this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving D. pneumosintes. DNA was extracted from the samples, and the presence of D. pneumosintes and 16 other bacterial species was determined by means of species-specific nested polymerase chain reaction. Positive and negative associations involving D. pneumosintes were investigated by computing the odds ratio of D. pneumosintes being found in a sample from endodontic infection in co-infection with one of the other target species. The association between the pairs containing D. pneumosintes and the occurrence of pain also was evaluated. D. pneumosintes was always detected in mixed infections with at least two of the other target species. D. pneumosintes was positively associated with Treponema denticola, Porphyromonas endodontalis, Fusobacterium nucleatum, Peptostreptococcus micros, Campylobacter rectus, Prevotella intermedia, T. pectinovorum, and T. vincentii. Negative associations were observed with Bacteroides forsythus, P. gingivalis, and Actinomyces israelii. No pair containing D. pneumosintes was found to be significantly associated with symptomatic cases (p > 0.01). The findings of this study lend considerable support to the notion of D. pneumosintes being an important endodontic pathogen, usually in a mixed infection. Positive associations of this species with other highly prevalent species, such as T. denticola and P. endodontalis, suggest that bacterial synergism can occur and thereby play an important role in the pathogenesis of different forms of periradicular lesions.
口腔纤毛菌是一种厌氧革兰氏阴性杆菌，最近被认为是一种潜在的牙髓病原体。在本研究中，对从伴有无症状或有症状根尖周病变的脓肿牙齿和感染根管中采集的样本进行检查，以确定是否存在涉及口腔纤毛菌的细菌组合。从样本中提取DNA，并通过种特异性巢式聚合酶链反应确定口腔纤毛菌和其他16种细菌的存在。通过计算在牙髓感染样本中与其他目标物种之一共同感染时发现口腔纤毛菌的优势比，研究了涉及口腔纤毛菌的正相关和负相关。还评估了包含口腔纤毛菌的配对与疼痛发生之间的关联。在与至少两种其他目标物种的混合感染中总是能检测到口腔纤毛菌。口腔纤毛菌与齿垢密螺旋体、牙髓卟啉单胞菌、具核梭杆菌、微小消化链球菌、直肠弯曲菌、中间普雷沃菌、果胶螺旋体菌和文森特螺旋体呈正相关。与福赛斯坦纳菌、牙龈卟啉单胞菌和以色列放线菌呈负相关。未发现包含口腔纤毛菌的配对与有症状病例有显著关联（p>0.01）。本研究结果为口腔纤毛菌通常在混合感染中是一种重要的牙髓病原体这一观点提供了相当多的支持。该物种与其他高度流行的物种（如齿垢密螺旋体和牙髓卟啉单胞菌）的正相关表明，细菌协同作用可能发生，从而在不同形式的根尖周病变的发病机制中发挥重要作用。
