Jaffe R I, Lowell G H, Gordon D M
Walter Reed Army Institute of Research, Washington, DC.
Am J Trop Med Hyg. 1990 Apr;42(4):309-13. doi: 10.4269/ajtmh.1990.42.309.
Three inbred mouse strains, C57BL/6 (H-2b), A/J (H-2a), and BALB/c (H-2d), and 1 outbred strain, CD-1, demonstrated differences in susceptibility to iv challenge with the ANKA clone of Plasmodium berghei. Mice were challenged with 100, 1,000, or 10,000 sporozoites, then evaluated daily beginning on day 4 for patency. CD-1 mice were further evaluated at challenge doses of 12,500, 25,000, and 50,000 sporozoites. C57BL/6 mice were the easiest to infect, with 90% becoming infected with 100 sporozoites. The outbred strain CD-1 was the most difficult to infect, requiring a challenge dose of 25,000 sporozoites/mouse in order to achieve a 100% infection rate. Mouse strains also demonstrated differences in their ability to be protected by intravenous immunization with gamma-irradiated sporozoites. A/J mice needed a minimum of 3 doses of irradiated sporozoites for protection against a challenge with 10,000 sporozoites. In contrast, BALB/c mice immunized with a single dose of 1,000 irradiated sporozoites are protected against a 10,000 sporozoite challenge. These data suggest that both infectivity and protection are genetically restricted and that susceptibility to infection may be inversely related to protection.
三种近交系小鼠品系，C57BL/6（H-2b）、A/J（H-2a）和BALB/c（H-2d），以及一种远交系品系CD-1，在对伯氏疟原虫ANKA克隆进行静脉内攻击的易感性方面表现出差异。用100、1000或10000个子孢子对小鼠进行攻击，然后从第4天开始每天评估其血中出现疟原虫的情况。对CD-1小鼠在12500、25000和50000个子孢子的攻击剂量下进行了进一步评估。C57BL/6小鼠最易感染，100个子孢子就能使90%的小鼠被感染。远交系品系CD-1最难感染，需要25000个子孢子/小鼠的攻击剂量才能达到100%的感染率。小鼠品系在用γ射线照射的子孢子进行静脉免疫时的受保护能力也表现出差异。A/J小鼠至少需要3剂照射过的子孢子才能抵御10000个子孢子的攻击。相比之下，用单剂量1000个照射过的子孢子免疫的BALB/c小鼠能够抵御10000个子孢子的攻击。这些数据表明，感染性和保护性都受到遗传限制，并且感染易感性可能与保护性呈负相关。
