Hass I, Müller S, Artoni R F, Sbalqueiro I J
Departamento de Genética, Universidade Federal do Paraná, Curitiba, Brasil.
Cytogenet Genome Res. 2011;135(1):42-50. doi: 10.1159/000330259. Epub 2011 Aug 16.
This work presents chromosome homology maps between Mus musculus (MMU) and 2 South American rodent species from the Cricetidae group: Necromys lasiurus (NLA, 2n = 34) and Thaptomys nigrita (TNI, 2n = 52), established by ZOO-FISH using mouse chromosome-specific painting probes. Extending previous molecular cytogenetic studies in Neotropical rodents, the purpose of this work was to delineate evolutionary chromosomal rearrangements in Cricetidae rodents and to reconstruct the phylogenetic relationships among the Akodontini species. Our phylogenetic reconstruction by maximum parsimony analysis of chromosomal characters confirmed one consistent clade of all Neotropical rodents studied so far. In both species analyzed here, we observed the syntenic association of chromosome segments homologous to MMU 8/13, suggesting that this chromosome form is a synapomorphic trait exclusive to Neotropical rodents. Further, the previously described Akodontini-specific syntenic associations MMU 3/18 and MMU 6/12 were observed in N.lasiurus but not in T. nigrita, although the latter species is considered a member of the Akodontini tribe by some authors. Finally, and in agreement with this finding, N.lasiurus and Akodon serrensis share the derived fission of MMU 13, which places them as basal sister clades within Akodontini.
这项工作展示了小家鼠（MMU）与仓鼠科的2种南美啮齿动物之间的染色体同源性图谱：黑髯鼠（NLA，2n = 34）和黑腹粗尾鼠（TNI，2n = 52），该图谱通过使用小鼠染色体特异性涂染探针的荧光原位杂交（ZOO-FISH）技术建立。扩展先前对新热带啮齿动物的分子细胞遗传学研究，这项工作的目的是描绘仓鼠科啮齿动物的进化染色体重排，并重建阿科东鼠属物种之间的系统发育关系。我们通过对染色体特征进行最大简约分析的系统发育重建，证实了迄今为止所有研究的新热带啮齿动物都有一个一致的进化枝。在本文分析的两个物种中，我们都观察到了与MMU 8/13同源的染色体片段的同线关联，这表明这种染色体形式是新热带啮齿动物独有的共衍征性状。此外，在黑髯鼠中观察到了先前描述的阿科东鼠属特异性同线关联MMU 3/18和MMU 6/12，而在黑腹粗尾鼠中未观察到，尽管一些作者认为后者是阿科东鼠属部落的成员。最后，与此发现一致的是，黑髯鼠和锯齿阿科东鼠共享MMU 13的衍生裂变，这使它们成为阿科东鼠属内的基部姐妹进化枝。
