Laboratório de Citogenética, Centro de Estudos Avançados da Biodiversidade, Instituto de Ciências Biológicas, Universidade Federal do Pará (UFPA), Belém, Pará, 66075-750, Brazil.
Cambridge Resource Centre for Comparative Genomics, Department of Veterinary Medicine, University of Cambridge, Cambridge 01223, UK.
Int J Mol Sci. 2020 Mar 31;21(7):2415. doi: 10.3390/ijms21072415.
Comparative chromosome-painting analysis among highly rearranged karyotypes of Sigmodontinae rodents (Rodentia, Cricetidae) detects conserved syntenic blocks, which are proposed as chromosomal signatures and can be used as phylogenetic markers. In the Akodontini tribe, the molecular topology (Cytb and/or IRBP) shows five low-supported clades (divisions: "", "", "", "", and "") within two high-supported major clades (clade A: "", "", and ""; clade B: "" and ""). Here, we examine the chromosomal signatures of the Akodontini tribe by using (HME) probes to study the karyotypes of (2n = 54, FN = 64) and (2n = 28, FN = 50), and compare these data with those from other taxa investigated using the same set of probes. We strategically employ the chromosomal signatures to elucidate phylogenetic relationships among the Akodontini. When we follow the evolution of chromosomal signature states, we find that the cytogenetic data corroborate the current molecular relationships in clade A nodes. We discuss the distinct events that caused karyotypic variability in the and genera. In addition, we propose that may constitute a species complex, and that the taxonomy should be revised to better delimit the geographical boundaries and their taxonomic status.
比较高度重排的 Sigmodontinae 啮齿动物（Rodentia，Cricetidae）的染色体绘画分析检测到保守的同线性块，这些块被提议作为染色体特征，并可作为系统发育标记。在 Akodontini 部落中，分子拓扑结构（Cytb 和/或 IRBP）显示出五个低支持的分支（分区：“”，“”，“”，“”和“”），内有两个高支持的主要分支（分支 A：“”，“”和“”；分支 B：“”和“”）。在这里，我们通过使用 （HME）探针研究了 Akodontini 部落的染色体特征，以研究 （2n = 54，FN = 64）和 （2n = 28，FN = 50）的染色体组型，并将这些数据与使用相同探针研究的其他分类群的数据进行比较。我们战略性地利用染色体特征来阐明 Akodontini 之间的系统发育关系。当我们遵循染色体特征状态的进化时，我们发现细胞遗传学数据证实了分支 A 节点中当前分子关系。我们讨论了导致 和 属中染色体变异性的独特事件。此外，我们提出 可能构成一个物种复合体，并且分类学应该进行修订，以更好地划定地理边界及其分类地位。
