Laboratory of Bioenergetics and Biochemical Toxicology, Department of Pesticide Chemistry & Technology, Faculty of Agriculture, Alexandria University, El-Chatby, 21545 Alexandria, Egypt.
J Bioenerg Biomembr. 2011 Oct;43(5):457-63. doi: 10.1007/s10863-011-9378-z. Epub 2011 Aug 17.
1,1-bis (p-chlorophenyl)-2, 2, 2-trichloroethane (DDT) has been used for control of malaria mosquitoes and other insect vectors of human diseases since 1945. Its use poses an environmental dilemma and efforts to replace it have been hampered by lack of information about its molecular target. This work identifies the 23 kDa band responsible for the DDT sensitivity in bees, as the OSCP and subunit "d" of the ATP synthase. The OSCP of the bee's ATP synthase contained 207 amino acids compared to 190 in bovine, which is insensitive to DDT, and the identities were only 47%. Subunit "d" of the bees had no counterpart in the bovine. Whether DDT is interacting only with OSCP, only with subunit "d", or with both subunits, remains to be assessed. Identification of the molecular target of DDT will lead the way to new target based insecticides aimed to protect plant, combat malaria and other insect transmitted diseases.
1,1-双（对氯苯基）-2,2,2-三氯乙烷（滴滴涕）自 1945 年以来一直被用于控制疟疾蚊子和其他人类疾病的昆虫媒介。它的使用带来了环境困境，由于缺乏关于其分子靶标的信息，因此难以替代它。这项工作确定了蜜蜂中负责滴滴涕敏感性的 23 kDa 带，即 OSCP 和 ATP 合酶的亚基“d”。与牛的 207 个氨基酸相比，蜜蜂 ATP 合酶的 OSCP 含有 207 个氨基酸，对滴滴涕不敏感，同一性仅为 47%。蜜蜂的亚基“d”在牛中没有对应物。滴滴涕是否仅与 OSCP 相互作用，仅与亚基“d”相互作用，还是与两个亚基都相互作用，还有待评估。滴滴涕的分子靶标的鉴定将为旨在保护植物、防治疟疾和其他昆虫传播疾病的新型基于靶标的杀虫剂开辟道路。
