Steen Hanno, Mann Matthias
Department of Systems Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
Nat Rev Mol Cell Biol. 2004 Sep;5(9):699-711. doi: 10.1038/nrm1468.
Proteomics is an increasingly powerful and indispensable technology in molecular cell biology. It can be used to identify the components of small protein complexes and large organelles, to determine post-translational modifications and in sophisticated functional screens. The key - but little understood - technology in mass-spectrometry-based proteomics is peptide sequencing, which we describe and review here in an easily accessible format.
蛋白质组学在分子细胞生物学中是一项日益强大且不可或缺的技术。它可用于识别小蛋白质复合物和大型细胞器的组成成分，确定翻译后修饰，并用于复杂的功能筛选。基于质谱的蛋白质组学中的关键技术——但却鲜为人知——是肽测序，我们在此以一种易于理解的形式对其进行描述和综述。
