State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Virol Sin. 2011 Aug;26(4):273-8. doi: 10.1007/s12250-011-3199-4. Epub 2011 Aug 17.
In order to develop an anti-FMDV A Type monoclonal antibody (mAb), BABL/c mice were immunized with FMDV A type. Monoclonal antibodies (mAbs) 7B11 and 8H4 against Foot-and-mouth disease virus (FMDV) serotype A were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with A/AV88. The microneutralization titer of the mAbs 7B11 and 8H4 were 1024 and 512, respectively. Both mAbs contain kappa light chains, the mAbs were IgG1. In order to define the mAbs binding epitopes, the reactivity of these mAbs against A Type FMDV, were examined using indirect ELISA, the result showed that both mAbs reacted with A Type FMDV. These mAbs may be used for further vaccine studies, diagnostic methods, prophylaxis, etiological and immunological research on FMDV. Characterization of these ncindicated that prepared anti-FMDV A mAbs had no cross-reactivity with Swine Vesicular Disease (SVD) or FMDV O, Asia1 and C Type antigens. Their titers in abdomen liquor were 1:5×10(6) and 1:2×10(6), respectively. 7B11 was found to be of subtype IgG(1), 8H4 was classified as IgG(2b) subtype. The mAbs prepared in this study, are specific for detection of FMDV serotype A, and is potentially useful for pen-side diagnosis.
为了研制抗口蹄疫病毒（FMDV）A型单克隆抗体（mAb），我们用 FMDV A 型对 BABL/c 小鼠进行了免疫接种。通过将 SP2/0 骨髓瘤细胞与用 A/AV88 免疫的小鼠的脾细胞融合，制备了针对 FMDV 血清型 A 的单克隆抗体 7B11 和 8H4。mAb 7B11 和 8H4 的微量中和效价分别为 1024 和 512。两种 mAb 均含有 κ 轻链，mAb 均为 IgG1。为了确定 mAb 的结合表位，我们使用间接 ELISA 检查了这些 mAb 对 A 型 FMDV 的反应性，结果表明两种 mAb 均与 A 型 FMDV 反应。这些 mAb 可用于进一步的疫苗研究、诊断方法、预防、口蹄疫病毒的病因学和免疫学研究。这些特性表明，制备的抗 FMDV A 型 mAb 与猪水疱病（SVD）或 FMDV O、亚洲 1 型和 C 型抗原无交叉反应性。它们在腹水中的效价分别为 1:5×10(6)和 1:2×10(6)。7B11 被发现为 IgG(1)亚型，8H4 被归类为 IgG(2b)亚型。本研究制备的 mAb 特异性检测 FMDV 血清型 A，具有潜在的现场诊断应用价值。
