Liu Wei, Wei Xiao, Yuan Jing, Huang Liuyu
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, China.
Sheng Wu Gong Cheng Xue Bao. 2011 Apr;27(4):539-45.
DNA stable-isotope probing (DNA-SIP) is a recently developed method with which the incorporation of stable isotope from a labeled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds. We summarize recent progress in SIP-metagenomic techniques and applications and discuss prospects for this combined approach in environmental microbiology and biotechnology.
DNA稳定同位素探测（DNA-SIP）是一种最近开发的方法，利用来自标记底物的稳定同位素掺入来鉴定环境中微生物的功能。该技术现已与宏基因组学结合使用，以建立微生物身份与特定代谢功能之间的联系。DNA-SIP与宏基因组学的结合不仅允许从宏基因组文库中检测罕见的低丰度物种，还有助于检测新的酶和生物活性化合物。我们总结了SIP-宏基因组技术和应用的最新进展，并讨论了这种联合方法在环境微生物学和生物技术中的前景。
