Department of Biological Sciences, the University of Warwick, Coventry CV4 7AL, UK.
Trends Microbiol. 2010 Apr;18(4):157-63. doi: 10.1016/j.tim.2010.02.002. Epub 2010 Mar 4.
The application of metagenomics, the culture-independent capture and subsequent analysis of genomic DNA from the environment, has greatly expanded our knowledge of the diversity of microbes and microbial protein families; however, the metabolic functions of many microorganisms remain largely unknown. DNA stable-isotope probing (DNA-SIP) is a recently developed method in which the incorporation of stable isotope from a labelled substrate is used to identify the function of microorganisms in the environment. The technique has now been used in conjunction with metagenomics to establish links between microbial identity and particular metabolic functions. The combination of DNA-SIP and metagenomics not only permits the detection of rare low-abundance species from metagenomic libraries but also facilitates the detection of novel enzymes and bioactive compounds.
宏基因组学的应用，即从环境中捕获和随后分析基因组 DNA 的非培养方法，极大地扩展了我们对微生物和微生物蛋白家族多样性的认识；然而，许多微生物的代谢功能仍然很大程度上未知。DNA 稳定同位素探针 (DNA-SIP) 是一种最近开发的方法，其中使用标记底物的稳定同位素掺入来鉴定环境中微生物的功能。该技术现已与宏基因组学结合使用，以建立微生物身份与特定代谢功能之间的联系。DNA-SIP 和宏基因组学的结合不仅允许从宏基因组文库中检测稀有低丰度物种，还促进了新酶和生物活性化合物的检测。
