Li Chunxia, Yan Daiyuan, Ni Jing, Guo Ziye, Cai Chun'er, He Peimin
Aqual-life Science and Technology College, Shanghai Ocean University, Shanghai 201306, China.
Sheng Wu Gong Cheng Xue Bao. 2011 Apr;27(4):614-9.
We developed large-scale preparation of phycoerythrin from Porphyra haitanensis, a main economic red algae in China. Firstly, P. haitanensis thallus was broken by using "swelling and smash" method. Then times of grads ammonium sulfate precipitation applied to the crude extraction were compared. Desalted solution was further purified with one-step chromatography using hydroxyapatite and properties on spectrum and molecular weight were identified finally. The results indicated that after four times of ammonium sulfate precipitation (15%, 50%, 10% and 40%), the absorption spectrum purity of P. haitanensis achieved 0.9 (A564/A280), and 507.82 mg phycoerythrin (A564/A280 > 3.2) was obtained from 7 kg fresh algae after further hydroxyapatite chromatography. This research provides a potential way for preparation of phycoerythrin in large sclae.
我们开展了从中国主要经济红藻坛紫菜中大规模制备藻红蛋白的研究。首先，采用“膨胀-捣碎”法破碎坛紫菜藻体。然后，比较了粗提物进行分级硫酸铵沉淀的次数。脱盐溶液再用羟基磷灰石进行一步层析进一步纯化，最后鉴定其光谱和分子量性质。结果表明，经4次硫酸铵沉淀（15%、50%、10%和40%）后，坛紫菜的吸收光谱纯度达到0.9（A564/A280），进一步经羟基磷灰石层析后，从7千克新鲜藻体中获得了507.82毫克藻红蛋白（A564/A280>3.2）。本研究为大规模制备藻红蛋白提供了一条潜在途径。
