Key Laboratory of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs, National Research and Development Center for Aquatic Product Processing, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, People's Republic of China.
J Sci Food Agric. 2023 Aug 30;103(11):5277-5287. doi: 10.1002/jsfa.12601. Epub 2023 Apr 17.
Oxidation has been reported as the one of the deterioration reactions of proteins in aquatic products. Searching for new bioactive substances from marine algae has been one of the main areas in food science and additives.
In this study, a novel protein from the red alga Porphyra haitanensis was determined after ammonium sulfate precipitation and gel filtration chromatography. It closely corresponded to the antioxidant activity and was identified as an uncharacterized protein with a molecular mass of 43 kDa, designated Ph43. Bioinformatic analysis revealed that Ph43 is a novel protein of non-phycobiliprotein family with putative chordin domains and rich in α-helical conformation. Recombinant protein (rPh43) was expressed in Escherichia coli as a Hig-tagged protein using a pET-22b vector system and purified by affinity high-performance liquid chromatography. Spectroscopy analysis revealed that there were no structural differences between rPh43 and natural recovered Ph43. Moreover, rPh43 showed equal/higher antioxidant activity compared with Ph43. rPh43 has the potential for application as a natural antioxidant for food stabilization.
Our results identified a novel antioxidant protein with molecular mass of 43 kDa derived from Porphyra haitanensis that belongs to the non-phycobiliprotein family. © 2023 Society of Chemical Industry.
氧化被认为是水产品中蛋白质劣化反应之一。从海洋藻类中寻找新的生物活性物质一直是食品科学和添加剂的主要领域之一。
在这项研究中，通过硫酸铵沉淀和凝胶过滤层析，从红藻 Porphyra haitanensis 中确定了一种新型蛋白质。它与抗氧化活性密切相关，被鉴定为一种分子量为 43 kDa 的未鉴定蛋白，命名为 Ph43。生物信息学分析表明，Ph43 是一种新型非藻胆蛋白家族的蛋白，具有假定的 Chordin 结构域，富含α-螺旋构象。使用 pET-22b 载体系统，将重组蛋白（rPh43）作为 Hig 标记蛋白在大肠杆菌中表达，并通过亲和高效液相色谱法进行纯化。光谱分析表明，rPh43 与天然回收的 Ph43 之间没有结构差异。此外，rPh43 表现出与 Ph43 相等/更高的抗氧化活性。rPh43 有可能作为一种天然抗氧化剂应用于食品稳定。
我们的结果鉴定了一种源自 Porphyra haitanensis 的新型分子量为 43 kDa 的抗氧化蛋白，属于非藻胆蛋白家族。 © 2023 化学工业协会。
