Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, People's Republic of China.
Nat Prod Res. 2011 Nov;25(19):1885-9. doi: 10.1080/14786419.2010.541395. Epub 2011 Aug 17.
Multidrug resistance (MDR) continues to be a major obstacle for successful anticancer therapy. In this work, fractions from 17 clinically used antitumour traditional Chinese medicinal herbs were tested for their potential to restore the sensitivity of MCF-7/ADR and A549/Taxol cells to a known antineoplastic agent. The effects of these fractions were evaluated by MTT method and an assay of the cellular accumulation of doxorubicin. Fractions from the PB group (herbs with the ability to promote blood circulation and remove blood stasis) showed more significant effects than fractions from the CH group (herbs with the ability to clear away heat and toxic materials). Fractions from CH₂Cl₂ extracts were more effective than fractions from EtOAc extracts. Five herbs (Curcuma wenyujin, Chrysanthemum indicum, Salvia chinensis, Ligusticum chuanxiong Hort. and Cassia tora L.) could sensitise these resistant cancer cells at a non-toxic concentration (10 µg mL⁻¹), and markedly increased doxorubicin accumulation in MCF-7/ADR cells, which necessitates further investigations on the active ingredients of these herbs and their underlying mechanisms.
多药耐药（MDR）仍然是癌症治疗成功的主要障碍。在这项工作中，测试了 17 种临床应用的抗肿瘤中药的馏分，以评估它们恢复 MCF-7/ADR 和 A549/Taxol 细胞对已知抗肿瘤药物敏感性的潜力。通过 MTT 法和阿霉素细胞积累测定评估这些馏分的作用。PB 组（具有活血化瘀作用的药物）的馏分比 CH 组（具有清热、解毒作用的药物）的馏分显示出更显著的效果。CH₂Cl₂提取物的馏分比 EtOAc 提取物的馏分更有效。五种草药（温郁金、野菊花、丹参、川芎和槐角）可以在非毒性浓度（10μgmL⁻¹）下使这些耐药癌细胞敏感，并显著增加 MCF-7/ADR 细胞中阿霉素的积累，这需要对这些草药的活性成分及其潜在机制进行进一步研究。
