Li Wei-Bing, Li Yang, Yu Chen, He Yong-Ming
Department of Integrated Traditional and Western Medicine, Jiangsu Cancer Hospital, Baizi Ting, No. 42, Nanjing, Jiangsu 210000, China.
Department of Radiation Oncology, Cancer Center, Sun Yat-sen University, Guangzhou, Guangdong 510006, China.
Evid Based Complement Alternat Med. 2015;2015:390812. doi: 10.1155/2015/390812. Epub 2015 Feb 2.
Yiqi Jianpi Huaji Decoction (YJHD), a traditional Chinese medicinal formula composed of twelve ingredients, has recently been reported to have a good clinical curative effect. The purpose of the present study was to evaluate the effects of YJHD on SGC7901/VCR gastric cancer cells and to elucidate the possible mechanism of action. First, the effects of a low dose of YJHD in combination with chemotherapeutic agents on SGC7901/VCR cells were assessed using the CCK-8 assay and flow cytometry, and the effects of YJHD on genes and proteins involved in drug resistance (MDR1, MRP, TUBB3, STMN1, and TS) were evaluated. Furthermore, transfection of SGC7901/VCR cells with siRNAs targeting these genes inhibited their expression, and the efficacy of vincristine against the cells was dramatically improved in vitro when these genes were silenced. These results demonstrate that low-dose YJHD inhibited cell proliferation, induced apoptosis, reversed MDR, and increased sensitivity to chemotherapeutic agents in vitro by downregulating P-gp, MRP, TUBB3, and STMN1 expression. MDR can be reversed by siRNAs targeting genes involved in MDR, and this strategy for cancer treatment should be evaluated in future studies.
益气健脾化瘀汤（YJHD）是一种由十二味药组成的中药方剂，最近有报道称其具有良好的临床疗效。本研究的目的是评估YJHD对SGC7901/VCR胃癌细胞的作用，并阐明其可能的作用机制。首先，使用CCK-8法和流式细胞术评估低剂量YJHD联合化疗药物对SGC7901/VCR细胞的影响，并评估YJHD对耐药相关基因和蛋白（MDR1、MRP、TUBB3、STMN1和TS）的影响。此外，用靶向这些基因的小干扰RNA（siRNA）转染SGC7901/VCR细胞可抑制其表达，当这些基因沉默时，长春新碱对细胞的体外疗效显著提高。这些结果表明，低剂量YJHD通过下调P-糖蛋白、MRP、TUBB3和STMN1的表达，在体外抑制细胞增殖、诱导凋亡、逆转多药耐药并增加对化疗药物的敏感性。靶向多药耐药相关基因的siRNA可逆转多药耐药，这种癌症治疗策略应在未来的研究中进行评估。
