Packialakshmi R M, Usha R
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.
Virus Genes. 2011 Dec;43(3):465-70. doi: 10.1007/s11262-011-0656-y. Epub 2011 Aug 18.
Vernonia yellow vein virus (VeYVV) is a distinct monopartite begomovirus associated with a satellite DNA β. After constructing dimers of both DNA A and DNA β in binary vectors, a number of infection methods were attempted. However, only a modified stem-prick method produced up to 83% infection in the natural host Vernonia cinerea, thus, fulfilling the Koch's postulate. The presence of the viral DNA in the agroinfected plants was confirmed by rolling circle amplification (RCA), followed by Southern hybridization. DNA β induces typical symptoms of Vernonia yellow vein disease (VeYVD) when co-agroinoculated with the begomovirus to Vernonia and also leads to the accumulation of DNA A systemically. VeYVV represents a new member of the emerging group of monopartite begomoviruses requiring a satellite component for symptom induction.
斑鸠菊黄脉病毒（VeYVV）是一种独特的单分体双生病毒，与卫星DNAβ相关。在二元载体中构建DNA A和DNAβ的二聚体后，尝试了多种感染方法。然而，只有改良的茎刺接种法在天然寄主灰叶斑鸠菊中产生了高达83%的感染率，从而满足了科赫法则。通过滚环扩增（RCA），随后进行Southern杂交，证实了农杆菌感染植物中病毒DNA的存在。当与双生病毒共同农杆菌接种到斑鸠菊中时，DNAβ会诱发斑鸠菊黄脉病（VeYVD）的典型症状，并且还会导致DNA A在体内系统性积累。VeYVV代表了新兴的单分体双生病毒组中的一个新成员，该组病毒需要卫星成分来诱导症状。
