Pogorelova T N, Linde V A, Gun'ko V O, Drukker N A
Klin Lab Diagn. 2011 Jun(6):10-4.
An ion exchange chromatographic technique was used to study the content of 18 free amino acids in the amniotic fluid of women with physiological and complicated pregnancy. The amino acid spectrum of amniotic fluid in placental insufficiency was found to be characterized by the lower content of arginine, serine, glycine, alanine, cysteine, tyrosine, phenylalanine, and glutamine, which are involved in many metabolic processes required to maintain biochemical homeostasis during gestation. There was an opposite tendency for dicarboxylic amino acids and histidine, the levels of which increased, by enhancing nitrogen imbalance. The degree of the found impairments of a number of amino acids considerably increased in case of birth of babies with brain lesions, which permits use of their amniotic fluid determination to predict the neonatal status.
采用离子交换色谱技术研究了生理妊娠和复杂妊娠女性羊水内18种游离氨基酸的含量。结果发现，胎盘功能不全时羊水的氨基酸谱特征为精氨酸、丝氨酸、甘氨酸、丙氨酸、半胱氨酸、酪氨酸、苯丙氨酸和谷氨酰胺含量较低，而这些氨基酸参与维持妊娠期生化稳态所需的许多代谢过程。二羧酸氨基酸和组氨酸则呈现相反趋势，其水平因氮失衡加剧而升高。若婴儿出生时伴有脑损伤，多种氨基酸的受损程度会显著增加，这使得通过检测羊水来预测新生儿状况成为可能。
