Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Str 24-25, D-14476 Golm, Germany.
Biomacromolecules. 2011 Oct 10;12(10):3753-60. doi: 10.1021/bm200991b. Epub 2011 Sep 6.
Polymer brushes on thiol-modified gold surfaces were synthesized by using terminal thiol groups for the surface-initiated free radical polymerization of methacrylic acid and dimethylaminoethyl methacrylate, respectively. Atomic force microscopy shows that the resulting poly(methacrylic acid) (PMAA) and poly(dimethylaminoethyl methacrylate) (PDMAEMA) brushes are homogeneous. Contact angle measurements show that the brushes are pH-responsive and can reversibly be protonated and deprotonated. Mineralization of the brushes with calcium phosphate at different pH yields homogeneously mineralized surfaces, and preosteoblastic cells proliferate on both the nonmineralized and mineralized surfaces. The number of living cells on the mineralized hybrid surfaces is ca. 3 times (PDMAEMA) and 10 times (PMAA) higher than on the corresponding nonmineralized brushes.
在巯基修饰的金表面上合成聚合物刷，分别使用末端巯基基团引发甲基丙烯酸和二甲氨基乙基甲基丙烯酸的自由基聚合。原子力显微镜显示，所得的聚（甲基丙烯酸）（PMAA）和聚（二甲氨基乙基甲基丙烯酸）（PDMAEMA）刷是均匀的。接触角测量表明，这些刷具有 pH 响应性，可以可逆地质子化和去质子化。在不同 pH 值下用磷酸钙对这些刷进行矿化，得到均匀矿化的表面，且前成骨细胞在非矿化和矿化表面上都能增殖。矿化杂化表面上活细胞的数量约为（PDMAEMA）和 10 倍（PMAA）高于相应的非矿化刷。
