Salbert G, Bailhache T, Zohar Y, Breton B, Jego P
Laboratoire de Physiologie des Régulations, U.A. CNRS 256, Université de Rennes I, France.
Gen Comp Endocrinol. 1990 Apr;78(1):110-22. doi: 10.1016/0016-6480(90)90052-n.
A rapid and sensitive heterologous enzyme-linked immunosorbent assay (ELISA) was developed to measure rainbow trout maturational gonadotropin. Purified salmon maturational gonadotropin (sGtH II) was used as reference hormone. Optimization of the procedure was performed by using an anti-beta sGtH serum. Two procedures were developed: an equilibrium assay (which did not involve a preincubation step) which lasted for 8 hr and a nonequilibrium assay (which involved a preincubation step) which lasted for 26 hr. The nonequilibrium assay gave the best sensitivity (70 pg/ml sample). GtH II measurements on in vivo and in vitro samples from GnRH analogs or sGnRH experiments showed that the ELISA procedure could be used over a wide range of concentrations. The method was validated by comparing GtH II concentrations measured by both RIA and ELISA.
开发了一种快速灵敏的异源酶联免疫吸附测定（ELISA）法来测定虹鳟成熟促性腺激素。纯化的鲑鱼成熟促性腺激素（sGtH II）用作参考激素。使用抗β sGtH血清对该方法进行优化。开发了两种方法：一种平衡测定法（不涉及预孵育步骤），持续8小时；一种非平衡测定法（涉及预孵育步骤），持续26小时。非平衡测定法具有最佳灵敏度（样品浓度为70 pg/ml）。对来自GnRH类似物或sGnRH实验的体内和体外样品进行的GtH II测量表明，ELISA方法可在广泛的浓度范围内使用。通过比较RIA和ELISA测定的GtH II浓度对该方法进行了验证。
