Shen Shuang, Wang Fengming, Chen Liwen, Wang Ting, Hu Yumin, Zhang Xueguang
Institute of Medical Biotechnology, Soochow University, 708 Renmin Road, Suzhou, China.
Hybridoma (Larchmt). 2011 Aug;30(4):361-8. doi: 10.1089/hyb.2011.0014.
Human inducible co-stimulator ligand (GL50, CD275), also known as B7-H2 or ICOSL, is a positive co-stimulatory molecule of B7/CD28 superfamily, which plays a critical role in immune response. Here we generated two novel mouse anti-human GL50 monoclonal antibodies (MAbs) using classical hybridoma technology. The two MAbs (clones 2B4 and 4D11) were IgG1 (κ) and IgG2a (κ), respectively, and bound specifically to human GL50. Epitope competition assay showed that 2B4 and 4D11 bind to the same epitope of GL50, which is not recognized by the commercially available GL50 MAb (9F-8A4). Functionally, the two MAbs act as a blocker of T cell proliferation. Taken together, as useful tools, these two antibodies might be of great value for further exploration of the immune identification and function of GL50.
人诱导性共刺激分子配体（GL50，CD275），也称为B7-H2或ICOSL，是B7/CD28超家族的一种正向共刺激分子，在免疫反应中起关键作用。在此，我们利用经典杂交瘤技术制备了两种新型小鼠抗人GL50单克隆抗体（MAb）。这两种单克隆抗体（克隆2B4和4D11）分别为IgG1（κ）和IgG2a（κ），并与人GL50特异性结合。表位竞争试验表明，2B4和4D11与GL50的同一表位结合，而市售的GL50单克隆抗体（9F-8A4）不识别该表位。在功能上，这两种单克隆抗体可作为T细胞增殖的阻滞剂。综上所述，作为有用的工具，这两种抗体对于进一步探索GL50的免疫识别和功能可能具有重要价值。
