Chen Jie, Wang Fengming, Cai Qiuping, Shen Shuang, Chen Youguo, Hao Chao, Sun Jing
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
Monoclon Antib Immunodiagn Immunother. 2013 Apr;32(2):125-31. doi: 10.1089/mab.2012.0121.
ICOSL, a newly identified member of the B7 superfamily, plays a major role in immune responses. In this study, a functional anti-human ICOSL monoclonal antibody (MAb) 3B3 was obtained and characterized by means of flow cytometry, Western blot, and competition assay. This MAb could specifically recognize a distinct epitope of the ICOSL molecule. As a functional antibody, MAb 3B3 could inhibit the proliferation of T lymphocytes stimulated by ICOSL-L929 transfectants. Furthermore, it could enhance IgG production of PWM-driven B cells. The results indicate that the ICOS-ICOSL signal is critically involved in specific humoral immunity.
诱导共刺激分子配体（ICOSL）是B7超家族新发现的成员，在免疫反应中起主要作用。在本研究中，获得了一种功能性抗人ICOSL单克隆抗体（MAb）3B3，并通过流式细胞术、蛋白质免疫印迹法和竞争试验对其进行了表征。该单克隆抗体能够特异性识别ICOSL分子的一个独特表位。作为一种功能性抗体，单克隆抗体3B3能够抑制由ICOSL-L929转染细胞刺激的T淋巴细胞增殖。此外，它还能增强PWM驱动的B细胞产生IgG。结果表明，ICOS-ICOSL信号在特异性体液免疫中起关键作用。
