黑色素瘤生长刺激活性的中性粒细胞激活特性。
Neutrophil-activating properties of the melanoma growth-stimulatory activity.
作者信息
Moser B, Clark-Lewis I, Zwahlen R, Baggiolini M
机构信息
Theodor-Kocher Institute, University of Bern, Switzerland.
出版信息
J Exp Med. 1990 May 1;171(5):1797-802. doi: 10.1084/jem.171.5.1797.
Abstract
Melanoma growth-stimulatory activity (MGSA), a peptide reported to be mitogenic for Hs294T human melanoma cells, has extensive sequence similarity to the neutrophil-activating peptide NAP-1/IL-8, suggesting functional similarities. To test this hypothesis, MGSA was chemically synthesized and tested for its effects on human neutrophils. It was found to induce chemotaxis, exocytosis of elastase, and changes in cytosolic-free calcium to an extent and at concentrations similar to NAP-1/IL-8. However, MGSA was considerably less potent than NAP-1/IL-8 in inducing the respiratory burst. Intradermal injections in rats of MGSA resulted in a massive accumulation of neutrophils. Our data demonstrate that, apart from its growth-stimulatory activity, MGSA is a potent inflammatory agonist with neutrophil-stimulating properties.
摘要
黑色素瘤生长刺激活性（MGSA）是一种据报道对Hs294T人黑色素瘤细胞有促有丝分裂作用的肽，它与中性粒细胞激活肽NAP-1/IL-8有广泛的序列相似性，提示功能上的相似性。为验证这一假说，化学合成了MGSA并检测其对人中性粒细胞的作用。发现它能诱导趋化作用、弹性蛋白酶的胞吐作用以及胞质游离钙的变化，其程度和浓度与NAP-1/IL-8相似。然而，在诱导呼吸爆发方面，MGSA的效力远低于NAP-1/IL-8。在大鼠皮内注射MGSA会导致中性粒细胞大量聚集。我们的数据表明，除了其生长刺激活性外，MGSA是一种具有中性粒细胞刺激特性的强效炎症激动剂。
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Anal Biochem. 1987 Sep;165(2):371-8. doi: 10.1016/0003-2697(87)90284-3.
2
3
4
Functional diversity of gro gene expression in human fibroblasts and mammary epithelial cells.人成纤维细胞和乳腺上皮细胞中gro基因表达的功能多样性
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9645-9. doi: 10.1073/pnas.85.24.9645.
5
Role of disulfide bridges in determining the biological activity of interleukin 3.二硫键在确定白细胞介素3生物活性中的作用。
Proc Natl Acad Sci U S A. 1988 Nov;85(21):7897-901. doi: 10.1073/pnas.85.21.7897.
6
Calcium fluxes and calcium buffering in human neutrophils.人类中性粒细胞中的钙通量与钙缓冲作用
J Biol Chem. 1986 Aug 5;261(22):10163-8.
7
Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3.用于造血细胞的蛋白质生长因子白细胞介素-3的自动化化学合成。
Science. 1986 Jan 10;231(4734):134-9. doi: 10.1126/science.3079915.
8
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