Anisowicz A, Bardwell L, Sager R
Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1987 Oct;84(20):7188-92. doi: 10.1073/pnas.84.20.7188.
Comparison by subtractive hybridization of mRNAs revealed a moderately abundant message in highly tumorigenic CHEF/16 cells present at very low levels in closely related nontumorigenic CHEF/18 cells. After cloning and sequencing the corresponding cDNA, computer comparison showed closest homology with the human connective tissue-activating peptide III (CTAP III). The human tumor cell cDNA hybridizing with the Chinese hamster clone was isolated, sequenced, and found to have closer similarity to the Chinese hamster gene than to CTAP III. Thus, the cloned cDNAs from Chinese hamster and human cells represent a different gene, named gro. Studies of its transcriptional regulation have shown that expression is tightly regulated by growth status in normal Chinese hamster and human cells and relaxed in the tumorigenic cells so far examined.
通过消减杂交法对mRNA进行比较,发现在高致瘤性的CHEF/16细胞中有中等丰度的信息,而在密切相关的非致瘤性CHEF/18细胞中该信息水平极低。在对相应的cDNA进行克隆和测序后,计算机比较显示其与人类结缔组织激活肽III(CTAP III)具有最接近的同源性。与中国仓鼠克隆杂交的人类肿瘤细胞cDNA被分离、测序,结果发现其与中国仓鼠基因的相似性比与CTAP III的相似性更高。因此,从中国仓鼠和人类细胞中克隆的cDNA代表了一个不同的基因,命名为gro。对其转录调控的研究表明,在正常中国仓鼠和人类细胞中,gro基因的表达受生长状态的严格调控,而在目前所检测的致瘤性细胞中其表达调控则较为松弛。