用于血清中对乙酰氨基酚测量的高度特异、敏感和快速的酶免疫分析法。

Highly specific, sensitive and rapid enzyme immunoassays for the measurement of acetaminophen in serum.

机构信息

Analytical Sciences Research Group, Pharmaceutical Science Research Division, School of Biomedical and Health Sciences, King's College London, University of London, 150 Stamford Street, London SE1 9NH, UK.

出版信息

Anal Bioanal Chem. 2011 Oct;401(7):2195-204. doi: 10.1007/s00216-011-5272-0. Epub 2011 Aug 19.

DOI:10.1007/s00216-011-5272-0

PMID:21853346

Abstract

Acetaminophen antibodies were purified using affinity chromatography and labelled with horseradish peroxidase (HRP). The antibody-HRP conjugate and a new acetaminophen derivative were used in the construction of two immunoassay methods facilitating the direct quantitative measurement of acetaminophen in serum: a 96-well microtitre plate and coated-tube ELISAs. A minimum detection limit of 0.2 μg mL(-1) and a dynamic range of 0.2 to 1 μg mL(-1) in serum were achieved using the 96-well microtitre plate ELISA. The tube assay was optimised for the measurement of the clinically critical acetaminophen concentration of 50 to 250 μg mL(-1) of serum. The quantitative and specific tests could be completed within less than an hour. Common drugs including aspirin showed less than 0.1% cross-reactivity.

摘要

采用亲和层析法纯化对乙酰氨基酚抗体，并将其标记辣根过氧化物酶（HRP）。抗体-HRP 结合物和一种新的对乙酰氨基酚衍生物用于构建两种免疫测定方法，可直接定量测量血清中的对乙酰氨基酚：96 孔微量滴定板和包被管 ELISA。使用 96 孔微量滴定板 ELISA 可实现血清中最小检测限为 0.2μg mL（-1）和动态范围为 0.2 至 1μg mL（-1）。管测定法针对血清中临床关键的对乙酰氨基酚浓度 50 至 250μg mL（-1）进行了优化。定量和特异性测试可在不到一个小时内完成。常见药物，包括阿司匹林，其交叉反应性小于 0.1%。