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用于检测牛尿液中对乙酰氨基酚（扑热息痛）的间接竞争侧向流动免疫分析方法的开发与验证

Development and validation of an indirect competitive lateral flow immunoassay for the detection of acetaminophen (paracetamol) in bovine urine.

作者信息

Sasse Samantha, Geballa-Koukoula Ariadni, Bovee Toine F H

机构信息

Wageningen Food Safety Research (WFSR), Part of Wageningen University & Research, Wageningen, The Netherlands.

出版信息

Anal Bioanal Chem. 2025 Mar;417(6):1177-1186. doi: 10.1007/s00216-024-05721-y. Epub 2025 Jan 7.

DOI:10.1007/s00216-024-05721-y
PMID:39777530
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11802664/
Abstract

Paracetamol (PCM) is a commonly used analgesic and antipyretic agent for humans worldwide. However, PCM overdoses or overuse can cause health issues, such as hepatoxicity. As PCM is also used for the treatment of farm animals, it is essential to monitor these residues in animal-derived matrices at risk-based sites in order to minimize the intake of PCM through the food chain. In the present study, we have developed a novel carbon nanoparticle (CNP)-based indirect competitive lateral flow immunoassay (icLFIA) for the rapid detection of PCM in bovine urine. The developed icLFIA can detect PCM residues within 10 min, and its performance was validated according to Commission Implementing Regulation (EU) 2021/808, i.e., determination of the detection capability (CCβ), specificity, robustness, and stability. The CCβ of the icLFIA for PCM in bovine urine is 5 mg/L and the icLFIA is proven to be selective and specific towards PCM in bovine urine, as no matrix interference and cross-reactivity were observed, except for high concentrations of orthocetamol. The icLFIA for PCM in bovine urine is robust to (small) variations in reading time, but it remains necessary to strictly use a dilution ratio of running buffer/bovine urine of 80/20. Moreover, the produced icLFIAs are stable for at least 56 days under the stored conditions. In conclusion, the developed and validated icLFIA provides a rapid and cost-effective method for on-site monitoring of PCM abuse in cattle.

摘要

对乙酰氨基酚（PCM）是全球范围内人类常用的止痛和解热剂。然而，PCM过量服用或过度使用会导致健康问题，如肝毒性。由于PCM也用于治疗农场动物，因此有必要在基于风险的场所监测动物源性基质中的这些残留，以尽量减少通过食物链摄入的PCM。在本研究中，我们开发了一种基于新型碳纳米颗粒（CNP）的间接竞争侧流免疫分析方法（icLFIA），用于快速检测牛尿液中的PCM。所开发的icLFIA能够在10分钟内检测到PCM残留，并且根据欧盟委员会实施条例（EU）2021/808对其性能进行了验证，即检测能力（CCβ）、特异性、稳健性和稳定性的测定。icLFIA对牛尿液中PCM的CCβ为5mg/L，并且已证明icLFIA对牛尿液中的PCM具有选择性和特异性，因为除了高浓度的邻乙酰氨基酚外，未观察到基质干扰和交叉反应。用于检测牛尿液中PCM的icLFIA对读数时间的（小）变化具有稳健性，但仍必须严格使用运行缓冲液/牛尿液80/20的稀释比例。此外，所生产的icLFIA在储存条件下至少56天内稳定。总之，所开发并经验证的icLFIA为现场监测牛群中PCM滥用情况提供了一种快速且经济高效的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/b21610475813/216_2024_5721_Fig8_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/4fbf9732b75d/216_2024_5721_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/fbf97c2fb651/216_2024_5721_Fig5_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/1fe9ac78a57c/216_2024_5721_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/b21610475813/216_2024_5721_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/9b84d4e6022c/216_2024_5721_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/92802a0f772b/216_2024_5721_Fig2_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/4fbf9732b75d/216_2024_5721_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/fbf97c2fb651/216_2024_5721_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/b07b26cb6974/216_2024_5721_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f52e/11802664/1fe9ac78a57c/216_2024_5721_Fig7_HTML.jpg
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