Department of Orthopaedics, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan.
J Orthop Res. 2012 Mar;30(3):401-7. doi: 10.1002/jor.21535. Epub 2011 Aug 18.
The objective of the present study was to investigate the effects of heat stimulation and glutamine (Gln) on the expression of extracellular matrix genes and heat shock protein 70 (HSP70) in rat articular cartilage in vivo and to determine whether HSP70 expression achieved with a combination of microwave (MW) and Gln suppresses osteoarthritis (OA) progression in a rat OA model. Stimulation at 40 W was assumed to be appropriate in the present study, and the effects of heat treatment at this intensity were evaluated. Articular cartilage was collected at 8 h after heat stimulation and/or intraarticular Gln administration, and total RNA was extracted. The expression of HSP70, aggrecan, and type II collagen was quantified using real-time RT-PCR. Cartilage samples from the OA model were subjected to hematoxylin and eosin (HE) and safranin O staining. HSP70 and aggrecan expression was greatest in a group receiving both MW and Gln. In the rat OA model, the severity of OA was significantly milder in a group receiving MW and Gln than in the control group. HSP70, stimulated by the combination of MW heat and Gln, may be involved in the suppression of OA progression.
本研究旨在探讨热刺激和谷氨酰胺（Gln）对体内大鼠关节软骨细胞外基质基因和热休克蛋白 70（HSP70）表达的影响，并确定微波（MW）与 Gln 联合应用诱导的 HSP70 表达是否能抑制大鼠骨关节炎（OA）模型中的 OA 进展。本研究假设 40 W 的刺激强度是合适的，并评估了该强度热疗的效果。在热刺激和/或关节内给予 Gln 后 8 h 采集关节软骨，提取总 RNA。采用实时 RT-PCR 定量检测 HSP70、聚集蛋白聚糖和 II 型胶原的表达。对 OA 模型的软骨样本进行苏木精和伊红（HE）及番红 O 染色。在同时接受 MW 和 Gln 治疗的组中，HSP70、聚集蛋白聚糖和 II 型胶原的表达最高。在大鼠 OA 模型中，与对照组相比，接受 MW 和 Gln 治疗的组中 OA 的严重程度明显较轻。MW 热和 Gln 联合刺激诱导的 HSP70 可能参与了 OA 进展的抑制。
