Department of Orthopaedic Surgery, Juntendo University Shizuoka Hospital, 1129 Nagaoka, Izunokuni, Shizuoka 410-2295, Japan.
J Orthop Res. 2010 Mar;28(3):361-9. doi: 10.1002/jor.20995.
In this study, the effect of low-intensity pulsed ultrasound (LIPUS) on cartilage was evaluated in a rat osteoarthritis (OA) model using serum biomarkers such as CTX-II (type II collagen degradation) and CPII (type II collagen synthesis) as well as histological criteria (Mankin score and immunohistochemical type II collagen staining). OA was surgically induced in the knee joint of rats by anterior cruciate/medial collateral ligament transection and medial meniscus resection (ACLT + MMx). Animals were divided into three groups: sham-operated group (Sham), ACLT + MMx group without LIPUS (-LIPUS), and ACLT + MMx group with LIPUS (+LIPUS; 30 mW/cm(2), 20 min/day for 28 days). CTX-II levels were elevated in both -LIPUS and +LIPUS groups compared to that in the Sham group after the operation, but there was no significant difference between +LIPUS and -LIPUS groups, suggesting that LIPUS does not affect the degradation of type II collagen in this model. In contrast, CPII was significantly increased in +LIPUS group compared to -LIPUS and Sham. Moreover, histological damage on the cartilage (Mankin score) was ameliorated by LIPUS, and type II collagen was immunohistochemically increased by LIPUS in the cartilage of an OA model. Of interest, mRNA expression of type II collagen was enhanced by LIPUS in chondrocytes. Together these observations suggest that LIPUS is likely to increase the type II collagen synthesis in articular cartilage, possibly via the activation of chondrocytes and induction of type II collagen mRNA expression, thereby exhibiting chondroprotective action in a rat OA model.
在这项研究中，我们使用血清生物标志物，如 CTX-II（II 型胶原降解）和 CPII（II 型胶原合成）以及组织学标准（Mankin 评分和 II 型胶原免疫组织化学染色），评估了低强度脉冲超声（LIPUS）对软骨的影响。通过前交叉韧带/内侧副韧带切断和内侧半月板切除术（ACLT+MMx）在大鼠膝关节中诱导 OA。动物分为三组：假手术组（Sham）、无 LIPUS 的 ACLT+MMx 组（-LIPUS）和有 LIPUS 的 ACLT+MMx 组（+LIPUS；30 mW/cm(2)，每天 20 分钟，共 28 天）。与 Sham 组相比，术后 -LIPUS 和 +LIPUS 组的 CTX-II 水平均升高，但 +LIPUS 和 -LIPUS 组之间无显著差异，提示 LIPUS 对该模型中 II 型胶原的降解无影响。相反，与 -LIPUS 和 Sham 组相比，+LIPUS 组的 CPII 显著增加。此外，LIPUS 改善了软骨的组织学损伤（Mankin 评分），并增加了 OA 模型软骨中 II 型胶原的免疫组织化学染色。有趣的是，LIPUS 增强了软骨细胞中 II 型胶原 mRNA 的表达。综上所述，这些观察结果表明，LIPUS 可能通过激活软骨细胞和诱导 II 型胶原 mRNA 表达，从而增加关节软骨中 II 型胶原的合成，在大鼠 OA 模型中表现出软骨保护作用。
