Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
Phytochem Anal. 2012 May-Jun;23(3):240-7. doi: 10.1002/pca.1349. Epub 2011 Aug 18.
Desmodium styracifolium, with C-flavone glycosides as main pharmacological effective compounds, is a popular Chinese medicinal herb and has been used to treat urination disturbance, urolithiasis, edema and jaundice. However, few systematic methods have been reported on the quality control of this natural herb.
To develop a method for control the quality of D. styracifolium by combining chromatographic fingerprints and major constituent quantification.
Separations were performed on an Ultimate XB-C-18 column by gradient elution using acetonitrile and 0.1% aqueous formic acid. Analytes were identified by HPLC coupled with electrospray ionisation mass spectrometry experiments.
Twenty common peaks in chromatographic fingerprints were first identified among 15 batches of D. styracifolium from various regions. On basis of this, a HPLC-PAD method was established to simultaneously quantify five major constituents, which was validated for limit of qualification, linearity and interday variation of precision and accuracy.
The assay developed could be considered as a suitable quality control method of D. styracifolium.
落花生藤以 C-黄酮糖苷为主要药理有效成分，是一种常用的中草药，用于治疗排尿障碍、尿路结石、水肿和黄疸。然而，对于这种天然草药的质量控制，报道的系统方法很少。
建立一种结合色谱指纹图谱和主要成分定量测定的方法来控制落花生藤的质量。
采用乙腈和 0.1%甲酸水溶液梯度洗脱，在 Ultimate XB-C-18 柱上进行分离。通过高效液相色谱-电喷雾电离质谱实验对分析物进行鉴定。
首先在来自不同地区的 15 批落花生藤中鉴定出色谱指纹图谱中的 20 个共有峰。在此基础上，建立了一种同时定量分析 5 种主要成分的 HPLC-PAD 方法，并对其进行了定量限、线性、日内精密度和准确度的验证。
所建立的测定方法可作为落花生藤的质量控制方法。
