Laboratorium voor Farmaceutische Biologie, Katholieke Universiteit Leuven, Belgium.
BJU Int. 2012 Jan;109(2):300-5. doi: 10.1111/j.1464-410X.2011.10465.x. Epub 2011 Aug 19.
To develop a diagnostic method relying on the preferential accumulation of a dye in non-muscle-invasive bladder cancer (NMIBC) that is visible in conjunction with white-light cystoscopy (WLC).
We investigated in detail the permeation of Evans blue in urothelial cell carcinoma (UCC) spheroids prepared from T24, J82 and RT-112 human cell lines and spheroids composed of normal human urothelial (NHU) cells. To gain more insight into the differential accumulation, all spheroids were investigated ultrastructurally using transmission electron microscopy (TEM).
We found that, after exposure to Evans blue for 2 h, UCC spheroids accumulated dramatically more dye than spheroids composed of NHU cells. Using TEM it was found that the malignant spheroids contain similar ultrastructural characteristics, i.e. a wide intercellular space and a decreased number of desmosome-like cell attachments, to those from clinical samples of non-papillary carcinoma in situ of the bladder.
We believe the present findings could be important for future developments in clinical diagnostics for early bladder cancer detection, staging and grading involving WLC.
开发一种诊断方法，依赖于染料在非肌肉浸润性膀胱癌（NMIBC）中的优先积累，这种染料在与白光膀胱镜检查（WLC）结合时是可见的。
我们详细研究了 Evans 蓝在 T24、J82 和 RT-112 人细胞系以及由正常人膀胱尿路上皮（NHU）细胞组成的球体中尿路上皮细胞癌（UCC）球体中的渗透。为了更深入地了解差异积累，我们使用透射电子显微镜（TEM）对所有球体进行了超微结构研究。
我们发现，暴露于 Evans 蓝 2 小时后，UCC 球体比由 NHU 细胞组成的球体积累了明显更多的染料。使用 TEM 发现，恶性球体具有与临床膀胱癌原位非乳头状癌样本相似的超微结构特征，即细胞间空间较宽，桥粒样细胞附着减少。
我们相信，这些发现对于未来涉及 WLC 的早期膀胱癌检测、分期和分级的临床诊断学的发展可能很重要。
