Department of Orthopaedics, 309th Hospital of PLA, Beijing 100091, China.
Cell Immunol. 2011;271(1):73-7. doi: 10.1016/j.cellimm.2011.06.007. Epub 2011 Jun 29.
The purpose of this study is to assess the potential of dendritic cells transfected with PD-L1 recombinant adenovirus induces CD8+ T cell suppression and kidney allograft tolerance. To prove it, DCs transfected with PD-L1 recombinant adenovirus (DC/Ad-PD-L1) were transferred into the MHC-mismatched rat kidney transplants. After kidney transplantation, the mixed lymphocyte reaction (MLR) assay and kidney function were analyzed. The results demonstrated that after administration of DC/Ad-PD-L1, the proliferation, cytokines secretion and activation marker expression of CD8+ T cells were suppressed. In addition, DC/Ad-PD-L1 could improve kidney function and survival of transplants. The findings suggested that DC/Ad-PD-L1 could induce CD8+ T cell tolerance and lead to kidney allograft tolerance, which provided a promising finding for clinical application.
本研究旨在评估转染 PD-L1 重组腺病毒的树突状细胞诱导 CD8+T 细胞抑制和肾移植耐受的潜力。为了证明这一点，将转染 PD-L1 重组腺病毒的树突状细胞（DC/Ad-PD-L1）转移到 MHC 错配大鼠肾移植中。肾移植后，分析混合淋巴细胞反应（MLR）测定和肾功能。结果表明，给予 DC/Ad-PD-L1 后，CD8+T 细胞的增殖、细胞因子分泌和激活标志物表达受到抑制。此外，DC/Ad-PD-L1 可改善移植肾的功能和存活。这些发现表明，DC/Ad-PD-L1 可诱导 CD8+T 细胞耐受并导致肾移植耐受，为临床应用提供了有希望的发现。
