Department of Plant Physiology and Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
J Plant Physiol. 2011 Nov 15;168(17):2021-7. doi: 10.1016/j.jplph.2011.06.015.
In the present study, we isolated novel tocochromanols from green leaves of Kalanchoe daigremontiana and primary leaves of etiolated seedlings of Phaseolus coccineus that were identified as β-, γ-, and δ-tocomonoenols with unsaturation at the terminal isoprene unit of the side chain. The content of γ-tocomonoenol in leaves of etiolated bean increased gradually with the age of seedlings, reaching 50% of the γ-tocopherol level in 40-day-old plants. The content of this compound in leaves was increased by short illumination of etiolated plants and by addition of homogentisic acid, a biosynthetic precursor of tocopherols. These data indicated that γ-tocomonoenol is synthesized de novo from homogentisic acid and tetrahydro-geranylgeraniol diphosphate, a phytol precursor. Based on these results, a biosynthetic pathway of tocomonoenols is proposed.
在本研究中，我们从大戟科的长寿花的绿叶和菜豆黄化幼苗的初生叶中分离到了新型生育三烯酚，它们被鉴定为β-、γ-和δ-生育单烯醇，其侧链末端异戊二烯单元不饱和。γ-生育单烯醇在黄化菜豆叶片中的含量随幼苗年龄的增加而逐渐增加，在 40 日龄的植株中达到γ-生育酚水平的 50%。短时间光照黄化植株和添加生育酚生物合成前体尿黑酸均可增加该化合物在叶片中的含量。这些数据表明，γ-生育单烯醇是从头合成的，来源于尿黑酸和植醇前体四氢香叶基二磷酸。基于这些结果，提出了生育单烯醇的生物合成途径。
